Detailed information of TA system TA058471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2758305..2759056
• Replicon: chromosome
• Accession: NZ_CP013338
• Organism: Raoultella ornithinolytica strain Yangling I2

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: ATN83_RS12850
• Protein ID: WP_064357053.1
• Coordinates: 2758574..2759056 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A808Y4Y8
• Locus tag: ATN83_RS12845
• Protein ID: WP_015584326.1
• Coordinates: 2758305..2758583 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATN83_RS12820	2753432..2753866	+	435	WP_032717454.1	GNAT family N-acetyltransferase	-
ATN83_RS12825	2754010..2754360	+	351	WP_032724357.1	hypothetical protein	-
ATN83_RS12830	2754713..2755213	+	501	WP_004862067.1	DUF1003 domain-containing protein	-
ATN83_RS12835	2755237..2757111	+	1875	WP_064357052.1	KUP/HAK/KT family potassium transporter	-
ATN83_RS12840	2757327..2757824	+	498	WP_048023692.1	hypothetical protein	-
ATN83_RS12845	2758305..2758583	+	279	WP_015584326.1	DUF1778 domain-containing protein	Antitoxin
ATN83_RS12850	2758574..2759056	+	483	WP_064357053.1	GNAT family N-acetyltransferase	Toxin
ATN83_RS12855	2759706..2760338	+	633	WP_001567369.1	hypothetical protein	-
ATN83_RS12860	2760367..2761770	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
ATN83_RS12865	2761989..2762621	+	633	WP_001567369.1	hypothetical protein	-
ATN83_RS12870	2762650..2764053	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2649884..2876455	226571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17640.54 Da
• Isoelectric Point: 9.7381

>T58471 WP_064357053.1 NZ_CP013338:2758574-2759056 [Raoultella ornithinolytica]
MGMRPPEPLSAGHNIAEFCCQDQVLNEWLKKKALKNHSTGISRVFVVCAENTKRVIAYYSLSSGSVHRNTVPGAYRRNAP
EAVPVIVLGRLAVDAAWVGKGLGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVREFYTRFGFEPSIINTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T58471 NZ_CP013338:2758574-2759056 [Raoultella ornithinolytica]
ATGGGAATGAGACCACCGGAACCACTCAGCGCTGGACACAACATAGCAGAATTTTGTTGTCAGGATCAGGTTCTTAACGA
GTGGTTGAAGAAGAAGGCGCTGAAGAACCACAGTACAGGCATTTCAAGGGTCTTTGTGGTCTGTGCCGAAAACACAAAAA
GGGTCATCGCGTATTACAGCTTGTCATCCGGGAGCGTGCACAGAAACACAGTACCGGGGGCATACCGTCGTAATGCACCT
GAAGCTGTACCGGTCATTGTTCTGGGAAGGCTTGCGGTCGATGCAGCCTGGGTAGGGAAGGGACTCGGAGCGGCACTGCT
TAAGGATGCAATATACCGTACAGAACATATCGCAGTTCAGGTCGGTGTAAGGGCGCTTCTCGTCCATGCCTTGAATGATG
AAGTCAGGGAGTTTTACACCAGGTTTGGATTCGAGCCCTCGATTATCAATACACTGACATTATTGTTCCCGATAAAAGTA
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10714.30 Da
• Isoelectric Point: 4.6722

>AT58471 WP_015584326.1 NZ_CP013338:2758305-2758583 [Raoultella ornithinolytica]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFASFMEELDAPMTAERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT58471 NZ_CP013338:2758305-2758583 [Raoultella ornithinolytica]
ATGAAACCAGACGTTCGTGAAGCTCCGATAAACATTCGGGCGAAAACATCTCAGCGCGACCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTTGAAGTGGCCTGTCGCGAAGCTCAGGACATTCTGTTGGATCAGCGCT
TGTTCCTGCTGGACGATAAGCAGTTTGCTAGTTTCATGGAAGAGCTTGACGCACCTATGACAGCGGAGCGTCAGGCACGT
ATTGATAACCTGATGAACCGCAAATCGCCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A808Y4Y8