Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33105..33652 Replicon chromosome
Accession NZ_CP013338
Organism Raoultella ornithinolytica strain Yangling I2

Toxin (Protein)


Gene name ccdB Uniprot ID A0A808XUV0
Locus tag ATN83_RS26985 Protein ID WP_048025274.1
Coordinates 33105..33413 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A808XYT1
Locus tag ATN83_RS00155 Protein ID WP_004869059.1
Coordinates 33416..33652 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATN83_RS00125 28284..28586 - 303 WP_004869065.1 PTS lactose/cellobiose transporter subunit IIA -
ATN83_RS00130 28721..30046 - 1326 WP_004869064.1 PTS cellobiose transporter subunit IIC -
ATN83_RS00135 30058..30372 - 315 WP_004869063.1 PTS sugar transporter subunit IIB -
ATN83_RS00140 30667..31608 + 942 WP_015585487.1 LacI family transcriptional regulator -
ATN83_RS00145 31703..31978 - 276 WP_160873538.1 hypothetical protein -
ATN83_RS00150 32087..32989 - 903 WP_015585496.1 EamA family transporter -
ATN83_RS26985 33105..33413 - 309 WP_048025274.1 CcdB family protein Toxin
ATN83_RS00155 33416..33652 - 237 WP_004869059.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ATN83_RS00160 33758..35191 - 1434 WP_004869058.1 PTS N-acetylmuramic acid transporter subunit IIBC -
ATN83_RS00165 35216..36118 - 903 WP_044350979.1 N-acetylmuramic acid 6-phosphate etherase -
ATN83_RS00170 36283..37446 - 1164 WP_064356565.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11778.62 Da        Isoelectric Point: 6.7156

>T58460 WP_048025274.1 NZ_CP013338:c33413-33105 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Download         Length: 309 bp

>T58460 NZ_CP013338:c33413-33105 [Raoultella ornithinolytica]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGGTCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGTCCGCGCGCCGATCGTTTAACACCAG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAACGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTTTGACGGGATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8898.08 Da        Isoelectric Point: 5.6579

>AT58460 WP_004869059.1 NZ_CP013338:c33652-33416 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT58460 NZ_CP013338:c33652-33416 [Raoultella ornithinolytica]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808XUV0

Antitoxin

Source ID Structure
AlphaFold DB A0A808XYT1

References