Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 188536..189272 Replicon plasmid pCAV1193-258
Accession NZ_CP013323
Organism Klebsiella pneumoniae strain CAV1193

Toxin (Protein)


Gene name tacT Uniprot ID -
Locus tag AGG09_RS27645 Protein ID WP_038992631.1
Coordinates 188536..189018 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag AGG09_RS27650 Protein ID WP_032415032.1
Coordinates 189006..189272 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGG09_RS27630 184111..185823 + 1713 WP_038992633.1 ROK family protein -
AGG09_RS27635 186271..186903 + 633 WP_001567369.1 hypothetical protein -
AGG09_RS27640 186932..188335 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AGG09_RS27645 188536..189018 - 483 WP_038992631.1 GNAT family N-acetyltransferase Toxin
AGG09_RS27650 189006..189272 - 267 WP_032415032.1 DUF1778 domain-containing protein Antitoxin
AGG09_RS27655 189717..189947 - 231 WP_004206660.1 hypothetical protein -
AGG09_RS27660 189961..190164 - 204 WP_004206661.1 hemolysin expression modulator Hha -
AGG09_RS27665 190225..190716 - 492 WP_004206662.1 hypothetical protein -
AGG09_RS27670 190850..192061 - 1212 WP_032415728.1 hypothetical protein -
AGG09_RS27675 192058..192306 - 249 WP_004193994.1 hypothetical protein -
AGG09_RS27680 192785..193462 + 678 WP_047718105.1 helix-turn-helix domain-containing protein -
AGG09_RS27685 193543..193953 + 411 WP_047718088.1 heme-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..257944 257944
- flank IS/Tn - - 186932..188335 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17351.06 Da        Isoelectric Point: 10.0704

>T58453 WP_038992631.1 NZ_CP013323:c189018-188536 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKSSQTHERTLFLKLP

Download         Length: 483 bp

>T58453 NZ_CP013323:c189018-188536 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGTCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10123.53 Da        Isoelectric Point: 4.5141

>AT58453 WP_032415032.1 NZ_CP013323:c189272-189006 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT58453 NZ_CP013323:c189272-189006 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCCCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References