Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 88895..89421 Replicon plasmid pCAV1193-258
Accession NZ_CP013323
Organism Klebsiella pneumoniae strain CAV1193

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AGG09_RS27115 Protein ID WP_000323025.1
Coordinates 89134..89421 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag AGG09_RS27110 Protein ID WP_004196370.1
Coordinates 88895..89134 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGG09_RS32280 84232..84424 - 193 Protein_93 MFS transporter -
AGG09_RS27085 84454..85431 + 978 WP_004196334.1 chromate resistance protein -
AGG09_RS32285 85388..86764 + 1377 Protein_95 chromate efflux transporter -
AGG09_RS27095 86795..87484 - 690 WP_004196322.1 hypothetical protein -
AGG09_RS27100 87498..88235 - 738 WP_008460272.1 HupE/UreJ family protein -
AGG09_RS31050 88279..88644 - 366 WP_009651956.1 hypothetical protein -
AGG09_RS27110 88895..89134 + 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AGG09_RS27115 89134..89421 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AGG09_RS27120 89488..90522 - 1035 Protein_101 IS481 family transposase -
AGG09_RS27125 90594..90752 + 159 WP_014343509.1 type I toxin-antitoxin system Hok family toxin -
AGG09_RS27130 90831..91943 + 1113 WP_001300563.1 IS4-like element IS421 family transposase -
AGG09_RS27135 92799..93071 + 273 WP_032425552.1 hypothetical protein -
AGG09_RS27140 93068..93418 + 351 WP_032425551.1 hypothetical protein -
AGG09_RS27145 93433..93750 + 318 WP_015065519.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..257944 257944


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T58452 WP_000323025.1 NZ_CP013323:89134-89421 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T58452 NZ_CP013323:89134-89421 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT58452 WP_004196370.1 NZ_CP013323:88895-89134 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp

>AT58452 NZ_CP013323:88895-89134 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References