Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 18819..19344 Replicon plasmid pCAV1193-258
Accession NZ_CP013323
Organism Klebsiella pneumoniae strain CAV1193

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7H9GHC5
Locus tag AGG09_RS26720 Protein ID WP_009309918.1
Coordinates 19039..19344 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag AGG09_RS26715 Protein ID WP_006788213.1
Coordinates 18819..19037 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGG09_RS26690 13886..14731 - 846 Protein_17 colicin V synthesis protein -
AGG09_RS26695 14721..15998 - 1278 WP_009309915.1 HlyD family secretion protein -
AGG09_RS26700 16660..17538 + 879 WP_009309916.1 restriction endonuclease -
AGG09_RS26705 17579..17995 - 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin -
AGG09_RS26710 17992..18222 - 231 WP_047718083.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AGG09_RS26715 18819..19037 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AGG09_RS26720 19039..19344 + 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB Toxin
AGG09_RS34145 19573..19713 + 141 WP_162898808.1 hypothetical protein -
AGG09_RS26730 19763..20098 + 336 WP_009309920.1 hypothetical protein -
AGG09_RS26735 20132..21148 + 1017 WP_009309921.1 hypothetical protein -
AGG09_RS26740 21346..22125 + 780 WP_023287113.1 site-specific integrase -
AGG09_RS26745 22183..22440 - 258 WP_009310077.1 hypothetical protein -
AGG09_RS33825 22569..22682 - 114 WP_014343462.1 hypothetical protein -
AGG09_RS26755 23214..24182 - 969 WP_000654811.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..257944 257944
- flank IS/Tn - - 12886..13854 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11571.24 Da        Isoelectric Point: 6.4661

>T58451 WP_009309918.1 NZ_CP013323:19039-19344 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T58451 NZ_CP013323:19039-19344 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGGTCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT58451 WP_006788213.1 NZ_CP013323:18819-19037 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT58451 NZ_CP013323:18819-19037 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H9GHC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References