Detailed information of TA system TA058450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 17579..18222
• Replicon: plasmid pCAV1193-258
• Accession: NZ_CP013323
• Organism: Klebsiella pneumoniae strain CAV1193

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A7D6UII7
• Locus tag: AGG09_RS26705
• Protein ID: WP_032425563.1
• Coordinates: 17579..17995 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: AGG09_RS26710
• Protein ID: WP_047718083.1
• Coordinates: 17992..18222 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGG09_RS26685	12886..13854	-	969	WP_014839937.1	IS5 family transposase	-
AGG09_RS26690	13886..14731	-	846	Protein_17	colicin V synthesis protein	-
AGG09_RS26695	14721..15998	-	1278	WP_009309915.1	HlyD family secretion protein	-
AGG09_RS26700	16660..17538	+	879	WP_009309916.1	restriction endonuclease	-
AGG09_RS26705	17579..17995	-	417	WP_032425563.1	type II toxin-antitoxin system VapC family toxin	Toxin
AGG09_RS26710	17992..18222	-	231	WP_047718083.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AGG09_RS26715	18819..19037	+	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
AGG09_RS26720	19039..19344	+	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	-
AGG09_RS34145	19573..19713	+	141	WP_162898808.1	hypothetical protein	-
AGG09_RS26730	19763..20098	+	336	WP_009309920.1	hypothetical protein	-
AGG09_RS26735	20132..21148	+	1017	WP_009309921.1	hypothetical protein	-
AGG09_RS26740	21346..22125	+	780	WP_023287113.1	site-specific integrase	-
AGG09_RS26745	22183..22440	-	258	WP_009310077.1	hypothetical protein	-
AGG09_RS33825	22569..22682	-	114	WP_014343462.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..257944	257944
	-	flank	IS/Tn	-	-	12886..13854	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.56 Da
• Isoelectric Point: 8.5403

>T58450 WP_032425563.1 NZ_CP013323:c17995-17579 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T58450 NZ_CP013323:c17995-17579 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8720.80 Da
• Isoelectric Point: 4.6050

>AT58450 WP_047718083.1 NZ_CP013323:c18222-17992 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAERKDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT58450 NZ_CP013323:c18222-17992 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTAAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A7D6UII7