Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7037..7680 Replicon plasmid pCAV1193-258
Accession NZ_CP013323
Organism Klebsiella pneumoniae strain CAV1193

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AGG09_RS26645 Protein ID WP_001044770.1
Coordinates 7037..7453 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AGG09_RS26650 Protein ID WP_001261282.1
Coordinates 7450..7680 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGG09_RS26625 2406..2756 + 351 WP_004187110.1 DUF305 domain-containing protein -
AGG09_RS26630 2942..3850 - 909 WP_032425603.1 HNH endonuclease -
AGG09_RS26635 4394..5416 - 1023 WP_000361404.1 helicase UvrD -
AGG09_RS26640 5401..6963 - 1563 WP_009309907.1 AAA family ATPase -
AGG09_RS26645 7037..7453 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AGG09_RS26650 7450..7680 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AGG09_RS33815 7637..7873 + 237 Protein_9 hypothetical protein -
AGG09_RS26660 7972..8565 + 594 WP_009309909.1 hypothetical protein -
AGG09_RS30940 8584..9024 + 441 WP_047057603.1 hypothetical protein -
AGG09_RS26665 9215..10151 - 937 Protein_12 CAAX protease -
AGG09_RS30945 10473..10814 + 342 WP_071844672.1 hypothetical protein -
AGG09_RS26675 10922..11557 - 636 WP_009309912.1 CPBP family intramembrane metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..257944 257944


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T58449 WP_001044770.1 NZ_CP013323:c7453-7037 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T58449 NZ_CP013323:c7453-7037 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT58449 WP_001261282.1 NZ_CP013323:c7680-7450 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT58449 NZ_CP013323:c7680-7450 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References