Detailed information of TA system TA058436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2152691..2153316
• Replicon: chromosome
• Accession: NZ_CP013322
• Organism: Klebsiella pneumoniae strain CAV1193

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AGG09_RS10705
• Protein ID: WP_047718441.1
• Coordinates: 2152691..2153074 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: AGG09_RS10710
• Protein ID: WP_004150355.1
• Coordinates: 2153074..2153316 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGG09_RS10690	2150057..2150959	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
AGG09_RS10695	2150956..2151591	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
AGG09_RS10700	2151588..2152517	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
AGG09_RS10705	2152691..2153074	-	384	WP_047718441.1	type II toxin-antitoxin system VapC family toxin	Toxin
AGG09_RS10710	2153074..2153316	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
AGG09_RS10715	2153521..2154438	+	918	WP_004187175.1	alpha/beta hydrolase	-
AGG09_RS10720	2154452..2155393	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
AGG09_RS10725	2155438..2155875	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AGG09_RS10730	2155872..2156732	-	861	WP_004146232.1	virulence factor BrkB family protein	-
AGG09_RS10735	2156726..2157325	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14350.59 Da
• Isoelectric Point: 7.3178

>T58436 WP_047718441.1 NZ_CP013322:c2153074-2152691 [Klebsiella pneumoniae]
MTSGSALFDTSILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T58436 NZ_CP013322:c2153074-2152691 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAGTATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT58436 WP_004150355.1 NZ_CP013322:c2153316-2153074 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT58436 NZ_CP013322:c2153316-2153074 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9