Detailed information of TA system TA058423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 399769..400297
• Replicon: chromosome
• Accession: NZ_CP013320
• Organism: Vibrio cholerae strain NCTC9420

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: ASZ86_RS16475
• Protein ID: WP_000221352.1
• Coordinates: 399769..400059 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: ASZ86_RS16480
• Protein ID: WP_000213183.1
• Coordinates: 400049..400297 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ86_RS16435	395082..395564	+	483	WP_001900268.1	hypothetical protein	-
ASZ86_RS16445	395718..396722	+	1005	WP_071919818.1	LD-carboxypeptidase	-
ASZ86_RS16450	396875..397321	+	447	WP_000128977.1	hypothetical protein	-
ASZ86_RS16455	397441..398370	+	930	WP_000335802.1	hypothetical protein	-
ASZ86_RS20505	398367..398477	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
ASZ86_RS16465	398505..398981	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
ASZ86_RS16470	399118..399633	+	516	WP_001201532.1	lipocalin family protein	-
ASZ86_RS16475	399769..400059	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ86_RS16480	400049..400297	-	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ASZ86_RS16490	400868..401521	+	654	WP_000036957.1	site-specific DNA-methyltransferase	-
ASZ86_RS16495	401793..402218	+	426	WP_000415748.1	hypothetical protein	-
ASZ86_RS16500	402426..402821	+	396	WP_000649527.1	DUF3465 domain-containing protein	-
ASZ86_RS16505	402972..403502	+	531	WP_000415563.1	hypothetical protein	-
ASZ86_RS16510	403640..404140	+	501	WP_000789723.1	hypothetical protein	-
ASZ86_RS16515	404296..404694	+	399	WP_001882293.1	hypothetical protein	-
ASZ86_RS16520	404794..405258	-	465	WP_000140244.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304702..436238	131536
	-	inside	Integron	-	-	309782..435862	126080

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T58423 WP_000221352.1 NZ_CP013320:c400059-399769 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T58423 NZ_CP013320:c400059-399769 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT58423 WP_000213183.1 NZ_CP013320:c400297-400049 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT58423 NZ_CP013320:c400297-400049 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8