Detailed information of TA system TA058422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 388785..389323
• Replicon: chromosome
• Accession: NZ_CP013320
• Organism: Vibrio cholerae strain NCTC9420

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q9KMJ0
• Locus tag: ASZ86_RS16380
• Protein ID: WP_000802136.1
• Coordinates: 388785..389084 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P58093
• Locus tag: ASZ86_RS16385
• Protein ID: WP_001107719.1
• Coordinates: 389081..389323 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ86_RS16330	384720..384902	+	183	WP_000923182.1	DUF645 family protein	-
ASZ86_RS16335	385102..385575	+	474	WP_001161076.1	GrpB family protein	-
ASZ86_RS16340	385590..385682	+	93	WP_014378730.1	DUF3265 domain-containing protein	-
ASZ86_RS16345	385724..386350	+	627	WP_000365424.1	LysE family translocator	-
ASZ86_RS16350	386473..387171	+	699	WP_001890502.1	hypothetical protein	-
ASZ86_RS16355	387181..387291	+	111	WP_079857360.1	DUF3265 domain-containing protein	-
ASZ86_RS16370	387869..388048	+	180	WP_001883058.1	DUF645 family protein	-
ASZ86_RS16375	388262..388657	+	396	WP_000870857.1	DUF3465 domain-containing protein	-
ASZ86_RS16380	388785..389084	-	300	WP_000802136.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ86_RS16385	389081..389323	-	243	WP_001107719.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
ASZ86_RS16390	389552..390130	+	579	WP_000110120.1	hypothetical protein	-
ASZ86_RS16395	390152..390244	+	93	WP_014164112.1	DUF3265 domain-containing protein	-
ASZ86_RS16405	390668..391351	+	684	WP_000877436.1	hypothetical protein	-
ASZ86_RS16410	391565..391831	+	267	WP_000937852.1	hypothetical protein	-
ASZ86_RS16415	391986..392585	+	600	WP_000429495.1	hypothetical protein	-
ASZ86_RS16420	392866..393801	+	936	WP_000051985.1	restriction endonuclease	-
ASZ86_RS16425	393767..393907	+	141	WP_001883049.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304702..436238	131536
	-	inside	Integron	-	-	309782..435862	126080

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11589.29 Da
• Isoelectric Point: 9.9232

>T58422 WP_000802136.1 NZ_CP013320:c389084-388785 [Vibrio cholerae]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T58422 NZ_CP013320:c389084-388785 [Vibrio cholerae]
ATGAAACCATTTAATCTTACCGTCGCCGCCAAAGCCGATTTACGTGATATTGCTTTATTCACTCAACGACGCTGGGGAAA
AGAGCAGCGAAATGTTTATTTAAAGCAATTCGATGATTCCTTTTGGCTTTTAGCGGAAAATCCCGACATTGGTAAATCAT
GCGATGAAATCCGAGAGGGATACAGAAAATTTCCCCAAGGGAGTCACGTCATCTTTTATCAGCAAACCGGCAGCCAACAA
ATCAGGGTGATCCGAATTCTTCATAAGAGCATGGATGTGAACCCAATATTCGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8964.84 Da
• Isoelectric Point: 4.1677

>AT58422 WP_001107719.1 NZ_CP013320:c389323-389081 [Vibrio cholerae]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLIEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT58422 NZ_CP013320:c389323-389081 [Vibrio cholerae]
ATGGCTAAAAATACAAGTATCACTCTTGGTGAACACTTCGATGGCTTTATTACAAGCCAAATACAAAGTGGGCGTTACGG
CTCAGCAAGTGAAGTCATTCGCTCTGCGCTACGTCTACTCGAAAACCAAGAAACCAAACTACAGTCACTCCGTCAACTAC
TTATTGAAGGAGAGCAAAGTGGTGACGCTGATTATGACCTTGATAGCTTCATCAATGAACTCGATAGTGAAAACATTCGA
TGA

Structures

Toxin

• PDB: 7R5A

Antitoxin

• AlphaFold DB: A0A151JFU4