Detailed information of TA system TA058420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 373108..373606
• Replicon: chromosome
• Accession: NZ_CP013320
• Organism: Vibrio cholerae strain NCTC9420

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: ASZ86_RS16205
• Protein ID: WP_000589156.1
• Coordinates: 373108..373374 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: ASZ86_RS16210
• Protein ID: WP_000643598.1
• Coordinates: 373361..373606 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ86_RS16160	368340..368876	+	537	WP_000644491.1	nucleotidyltransferase family protein	-
ASZ86_RS20780	368995..369156	+	162	WP_000481782.1	hypothetical protein	-
ASZ86_RS16165	369345..369455	+	111	WP_071908335.1	DUF3265 domain-containing protein	-
ASZ86_RS16170	369507..369860	+	354	WP_000384364.1	hypothetical protein	-
ASZ86_RS16175	370072..370314	+	243	WP_000107461.1	hypothetical protein	-
ASZ86_RS16180	370566..370844	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
ASZ86_RS20785	371219..371362	+	144	Protein_366	DUF645 family protein	-
ASZ86_RS20790	371416..371454	+	39	WP_082798268.1	hypothetical protein	-
ASZ86_RS16195	371666..372133	+	468	WP_001289288.1	OsmC family protein	-
ASZ86_RS16200	372261..372923	+	663	WP_000960654.1	hypothetical protein	-
ASZ86_RS16205	373108..373374	+	267	WP_000589156.1	BrnT family toxin	Toxin
ASZ86_RS16210	373361..373606	+	246	WP_000643598.1	hypothetical protein	Antitoxin
ASZ86_RS16215	373702..374496	+	795	WP_001911581.1	hypothetical protein	-
ASZ86_RS20795	374599..374727	+	129	WP_071908338.1	general secretion pathway protein GspI	-
ASZ86_RS16230	374788..374970	+	183	WP_000923182.1	DUF645 family protein	-
ASZ86_RS16235	375186..376190	+	1005	WP_000964920.1	LD-carboxypeptidase	-
ASZ86_RS16240	376343..376702	+	360	WP_001071541.1	VOC family protein	-
ASZ86_RS16245	376975..377208	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
ASZ86_RS16250	377476..377982	+	507	WP_000393074.1	hypothetical protein	-
ASZ86_RS16255	378139..378525	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304702..436238	131536
	-	inside	Integron	-	-	309782..435862	126080

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T58420 WP_000589156.1 NZ_CP013320:373108-373374 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T58420 NZ_CP013320:373108-373374 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT58420 WP_000643598.1 NZ_CP013320:373361-373606 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT58420 NZ_CP013320:373361-373606 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2