Detailed information of TA system TA058334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 753663..754161
• Replicon: chromosome
• Accession: NZ_CP013312
• Organism: Vibrio cholerae strain E1320

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: ASZ88_RS17710
• Protein ID: WP_000589156.1
• Coordinates: 753663..753929 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: ASZ88_RS17715
• Protein ID: WP_000643598.1
• Coordinates: 753916..754161 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ88_RS17665	748895..749431	+	537	WP_000644491.1	nucleotidyltransferase family protein	-
ASZ88_RS21150	749550..749711	+	162	WP_000481782.1	hypothetical protein	-
ASZ88_RS17670	749900..750010	+	111	WP_071908335.1	DUF3265 domain-containing protein	-
ASZ88_RS17675	750062..750415	+	354	WP_000384364.1	hypothetical protein	-
ASZ88_RS17680	750627..750869	+	243	WP_000107461.1	hypothetical protein	-
ASZ88_RS17685	751121..751399	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
ASZ88_RS21155	751774..751917	+	144	Protein_711	DUF645 family protein	-
ASZ88_RS21160	751971..752009	+	39	WP_082798268.1	hypothetical protein	-
ASZ88_RS17700	752221..752688	+	468	WP_001289288.1	OsmC family protein	-
ASZ88_RS17705	752816..753478	+	663	WP_000960654.1	hypothetical protein	-
ASZ88_RS17710	753663..753929	+	267	WP_000589156.1	BrnT family toxin	Toxin
ASZ88_RS17715	753916..754161	+	246	WP_000643598.1	hypothetical protein	Antitoxin
ASZ88_RS17720	754257..755051	+	795	WP_001911581.1	hypothetical protein	-
ASZ88_RS17735	755343..755525	+	183	WP_000923182.1	DUF645 family protein	-
ASZ88_RS17740	755741..756745	+	1005	WP_000964920.1	LD-carboxypeptidase	-
ASZ88_RS17750	757033..757215	+	183	WP_000923182.1	DUF645 family protein	-
ASZ88_RS17755	757412..757837	+	426	WP_000403005.1	GNAT family N-acetyltransferase	-
ASZ88_RS17760	757977..758567	+	591	WP_000021480.1	hypothetical protein	-
ASZ88_RS17765	758721..759080	+	360	WP_001071529.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	688711..843534	154823
	-	inside	Integron	catB9	-	693791..843158	149367

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T58334 WP_000589156.1 NZ_CP013312:753663-753929 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T58334 NZ_CP013312:753663-753929 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT58334 WP_000643598.1 NZ_CP013312:753916-754161 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT58334 NZ_CP013312:753916-754161 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2