Detailed information of TA system TA058297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): dhiT-phd/-
• Location: 446799..447203
• Replicon: chromosome
• Accession: NZ_CP013308
• Organism: Vibrio cholerae strain E506

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: ASZ84_RS16790
• Protein ID: WP_001114075.1
• Coordinates: 446934..447203 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: ASZ84_RS20165
• Protein ID: WP_099607150.1
• Coordinates: 446799..446903 (+)
• Length: 35 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ84_RS16740	442268..442555	+	288	WP_000426470.1	hypothetical protein	-
ASZ84_RS16745	442696..443067	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ84_RS16750	443134..443559	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
ASZ84_RS16755	443556..444089	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
ASZ84_RS16760	444291..444548	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ84_RS16765	444536..444838	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ84_RS16775	445109..445993	+	885	WP_000842472.1	DUF3800 domain-containing protein	-
ASZ84_RS16780	446143..446742	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
ASZ84_RS20165	446799..446903	+	105	WP_099607150.1	acetyltransferase	Antitoxin
ASZ84_RS16790	446934..447203	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
ASZ84_RS16795	447197..447718	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
ASZ84_RS20170	448017..448142	+	126	WP_001905700.1	DUF645 family protein	-
ASZ84_RS16805	448393..448788	+	396	WP_001000867.1	hypothetical protein	-
ASZ84_RS16810	448927..449205	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
ASZ84_RS16815	449202..449486	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
ASZ84_RS16820	449680..450195	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
ASZ84_RS16825	450379..450792	+	414	WP_000049420.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304088..451655	147567
	-	inside	Integron	-	-	309168..451279	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T58297 WP_001114075.1 NZ_CP013308:446934-447203 [Vibrio cholerae]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T58297 NZ_CP013308:446934-447203 [Vibrio cholerae]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin

Protein

• Length: 35 a.a.
• Molecular weight: 3991.79 Da
• Isoelectric Point: 9.2853

>AT58297 WP_099607150.1 NZ_CP013308:446799-446903 [Vibrio cholerae]
VVSVVVFEFSSMRCQPLRRALYAYRNCLLKDTLL

Nucleotide

• Length: 105 bp

>AT58297 NZ_CP013308:446799-446903 [Vibrio cholerae]
GTGGTTTCGGTTGTTGTGTTTGAGTTTAGTAGTATGCGCTGCCAGCCCCTTAGGCGGGCGTTATATGCTTATAGGAATTG
TCTCCTAAAGGATACGCTGCTATAG

Structures

Toxin

• AlphaFold DB: A0A086SLD6