Detailed information of TA system TA058295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 443134..444089
• Replicon: chromosome
• Accession: NZ_CP013308
• Organism: Vibrio cholerae strain E506

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: ASZ84_RS16755
• Protein ID: WP_000118351.1
• Coordinates: 443556..444089 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ASZ84_RS16750
• Protein ID: WP_001882332.1
• Coordinates: 443134..443559 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ84_RS16695	438664..438915	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ84_RS16700	438905..439192	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ84_RS16705	439320..439775	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ84_RS16715	440097..440249	+	153	WP_001884520.1	DUF645 family protein	-
ASZ84_RS16720	440451..440948	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ84_RS16725	440945..441217	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ84_RS16735	441448..442116	+	669	WP_000043871.1	hypothetical protein	-
ASZ84_RS16740	442268..442555	+	288	WP_000426470.1	hypothetical protein	-
ASZ84_RS16745	442696..443067	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ84_RS16750	443134..443559	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
ASZ84_RS16755	443556..444089	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
ASZ84_RS16760	444291..444548	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ84_RS16765	444536..444838	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ84_RS16775	445109..445993	+	885	WP_000842472.1	DUF3800 domain-containing protein	-
ASZ84_RS16780	446143..446742	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
ASZ84_RS20165	446799..446903	+	105	WP_099607150.1	acetyltransferase	-
ASZ84_RS16790	446934..447203	+	270	WP_001114075.1	DUF4160 domain-containing protein	-
ASZ84_RS16795	447197..447718	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
ASZ84_RS20170	448017..448142	+	126	WP_001905700.1	DUF645 family protein	-
ASZ84_RS16805	448393..448788	+	396	WP_001000867.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304088..451655	147567
	-	inside	Integron	-	-	309168..451279	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T58295 WP_000118351.1 NZ_CP013308:443556-444089 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T58295 NZ_CP013308:443556-444089 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT58295 WP_001882332.1 NZ_CP013308:443134-443559 [Vibrio cholerae]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT58295 NZ_CP013308:443134-443559 [Vibrio cholerae]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94