Detailed information of TA system TA058290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 417958..418486
• Replicon: chromosome
• Accession: NZ_CP013308
• Organism: Vibrio cholerae strain E506

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: ASZ84_RS16475
• Protein ID: WP_000221352.1
• Coordinates: 417958..418248 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: ASZ84_RS16480
• Protein ID: WP_000213183.1
• Coordinates: 418238..418486 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ84_RS20460	413515..413640	+	126	WP_001944767.1	DUF645 family protein	-
ASZ84_RS20465	413656..413694	+	39	WP_106019119.1	hypothetical protein	-
ASZ84_RS16450	413907..414911	+	1005	WP_000964925.1	LD-carboxypeptidase	-
ASZ84_RS16455	415064..415510	+	447	WP_000128977.1	hypothetical protein	-
ASZ84_RS16460	415630..416559	+	930	WP_000335802.1	hypothetical protein	-
ASZ84_RS20115	416556..416666	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
ASZ84_RS16465	416694..417170	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
ASZ84_RS16470	417307..417822	+	516	WP_001201532.1	lipocalin family protein	-
ASZ84_RS16475	417958..418248	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ84_RS16480	418238..418486	-	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ASZ84_RS16495	419057..419710	+	654	WP_000036957.1	site-specific DNA-methyltransferase	-
ASZ84_RS16500	419982..420407	+	426	WP_000415748.1	hypothetical protein	-
ASZ84_RS16505	420615..421010	+	396	WP_000649527.1	DUF3465 domain-containing protein	-
ASZ84_RS16510	421161..421691	+	531	WP_000415563.1	hypothetical protein	-
ASZ84_RS16515	421829..422329	+	501	WP_000789723.1	hypothetical protein	-
ASZ84_RS16520	422485..422883	+	399	WP_001882293.1	hypothetical protein	-
ASZ84_RS16525	422983..423447	-	465	WP_000140244.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304088..451655	147567
	-	inside	Integron	-	-	309168..451279	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T58290 WP_000221352.1 NZ_CP013308:c418248-417958 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T58290 NZ_CP013308:c418248-417958 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT58290 WP_000213183.1 NZ_CP013308:c418486-418238 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT58290 NZ_CP013308:c418486-418238 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8