Detailed information of TA system TA058287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 366948..367446
• Replicon: chromosome
• Accession: NZ_CP013308
• Organism: Vibrio cholerae strain E506

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: ASZ84_RS15945
• Protein ID: WP_000589156.1
• Coordinates: 366948..367214 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: ASZ84_RS15950
• Protein ID: WP_000643598.1
• Coordinates: 367201..367446 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ84_RS19970	362596..362756	+	161	Protein_340	DUF3709 domain-containing protein	-
ASZ84_RS15895	362777..362998	+	222	WP_012705854.1	DUF3709 domain-containing protein	-
ASZ84_RS15905	363318..363638	+	321	WP_001909340.1	DUF1778 domain-containing protein	-
ASZ84_RS15910	363635..364168	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
ASZ84_RS15915	364406..364684	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
ASZ84_RS20420	365059..365202	+	144	Protein_345	DUF645 family protein	-
ASZ84_RS20425	365256..365294	+	39	WP_082798268.1	hypothetical protein	-
ASZ84_RS15935	365506..365973	+	468	WP_001289288.1	OsmC family protein	-
ASZ84_RS15940	366101..366763	+	663	WP_000960654.1	hypothetical protein	-
ASZ84_RS15945	366948..367214	+	267	WP_000589156.1	BrnT family toxin	Toxin
ASZ84_RS15950	367201..367446	+	246	WP_000643598.1	hypothetical protein	Antitoxin
ASZ84_RS15955	367542..368336	+	795	WP_001911581.1	hypothetical protein	-
ASZ84_RS15960	368494..369498	+	1005	WP_000964920.1	LD-carboxypeptidase	-
ASZ84_RS15970	369786..369968	+	183	WP_000923182.1	DUF645 family protein	-
ASZ84_RS15975	370165..370590	+	426	WP_000403005.1	GNAT family N-acetyltransferase	-
ASZ84_RS15980	370730..371320	+	591	WP_000021480.1	hypothetical protein	-
ASZ84_RS15985	371474..371833	+	360	WP_001071529.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304088..451655	147567
	-	inside	Integron	-	-	309168..451279	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T58287 WP_000589156.1 NZ_CP013308:366948-367214 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T58287 NZ_CP013308:366948-367214 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT58287 WP_000643598.1 NZ_CP013308:367201-367446 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT58287 NZ_CP013308:367201-367446 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2