Detailed information of TA system TA058284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 336272..336810
• Replicon: chromosome
• Accession: NZ_CP013308
• Organism: Vibrio cholerae strain E506

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A0K9UM73
• Locus tag: ASZ84_RS15620
• Protein ID: WP_000802138.1
• Coordinates: 336272..336571 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A271VK51
• Locus tag: ASZ84_RS15625
• Protein ID: WP_001107720.1
• Coordinates: 336568..336810 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ84_RS15575	331615..331767	+	153	WP_001884520.1	DUF645 family protein	-
ASZ84_RS15580	331969..332457	+	489	WP_016710990.1	hypothetical protein	-
ASZ84_RS15585	332594..333070	+	477	WP_001894984.1	GNAT family N-acetyltransferase	-
ASZ84_RS20240	333217..333753	+	537	WP_001083622.1	hypothetical protein	-
ASZ84_RS19905	333850..334011	+	162	Protein_294	DUF3709 domain-containing protein	-
ASZ84_RS15595	334005..334265	+	261	Protein_295	DUF3709 domain-containing protein	-
ASZ84_RS15605	334542..335558	+	1017	WP_001894976.1	hypothetical protein	-
ASZ84_RS15610	335703..336155	+	453	WP_000863110.1	GNAT family N-acetyltransferase	-
ASZ84_RS15620	336272..336571	-	300	WP_000802138.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ84_RS15625	336568..336810	-	243	WP_001107720.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
ASZ84_RS15630	337077..337790	+	714	WP_000123660.1	Fic family protein	-
ASZ84_RS15635	337978..338493	+	516	WP_000284463.1	hypothetical protein	-
ASZ84_RS15645	338612..339460	+	849	WP_000472050.1	hypothetical protein	-
ASZ84_RS19910	339445..339573	+	129	WP_088126095.1	DUF3265 domain-containing protein	-
ASZ84_RS15650	339610..340386	+	777	WP_001894915.1	hypothetical protein	-
ASZ84_RS15655	340547..341167	+	621	WP_001173624.1	hypothetical protein	-
ASZ84_RS15665	341694..341810	+	117	WP_080314581.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304088..451655	147567
	-	inside	Integron	-	-	309168..451279	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11617.34 Da
• Isoelectric Point: 9.8184

>T58284 WP_000802138.1 NZ_CP013308:c336571-336272 [Vibrio cholerae]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKYPQGSHVIFYQQIGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T58284 NZ_CP013308:c336571-336272 [Vibrio cholerae]
ATGAAACCATTTAATCTTACTGTCGCCGCTAAAGCCGATTTACGGGATATTGCTTTATTCACTCAACGACGCTGGGGAAA
AGAGCAGCGAAATGTTTATTTAAAGCAATTCGATGATTCCTTTTGGCTTTTAGCGGAAAATCCCGACATCGGTAAATCAT
GCGATGAAATTCGAGAGGGATACAGAAAATATCCCCAAGGGAGCCACGTCATCTTTTATCAGCAAATCGGCAGCCAACAA
ATCAGGGTGATCCGAATTCTTCATAAGAGCATGGATGTGAACCCAATATTTGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.82 Da
• Isoelectric Point: 4.1677

>AT58284 WP_001107720.1 NZ_CP013308:c336810-336568 [Vibrio cholerae]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLVEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT58284 NZ_CP013308:c336810-336568 [Vibrio cholerae]
ATGGCTAAAAACACAAGTATCACTCTTGGTGAACACTTCGATGGCTTTATTACAAGCCAAATACAAAGTGGACGTTACGG
CTCAGCAAGTGAAGTCATTCGCTCTGCGCTACGTCTACTCGAAAACCAAGAAACCAAACTACAGTCACTCCGTCAATTAC
TTGTTGAAGGTGAGCAAAGTGGTGATGCTGATTATGACCTTGATAGCTTCATCAATGAACTCGATAGTGAAAACATTCGA
TGA

Structures

Toxin

• AlphaFold DB: A0A0K9UM73

Antitoxin

• AlphaFold DB: A0A271VK51