Detailed information of TA system TA058281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 444592..446417
• Replicon: chromosome
• Accession: NZ_CP013307
• Organism: Vibrio cholerae strain E506

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: ASZ84_RS02065
• Protein ID: WP_000392003.1
• Coordinates: 445056..446417 (-)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: ASZ84_RS02060
• Protein ID: WP_000804849.1
• Coordinates: 444592..445059 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ84_RS02045	439872..440576	-	705	WP_001880605.1	NERD domain-containing protein	-
ASZ84_RS02050	440646..443582	-	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
ASZ84_RS02055	443586..444164	-	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
ASZ84_RS02060	444592..445059	-	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
ASZ84_RS02065	445056..446417	-	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
ASZ84_RS02075	447021..447998	+	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
ASZ84_RS02080	448024..448704	-	681	Protein_370	IS5 family transposase	-
ASZ84_RS02085	449046..450566	-	1521	WP_000640055.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T58281 WP_000392003.1 NZ_CP013307:c446417-445056 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T58281 NZ_CP013307:c446417-445056 [Vibrio cholerae]
ATGGAAGAGCTAACCGTACAAGCTTTTATCAGAGGTGAATGGATAGATATTGGTATCATCTCATTCCCTAAAAGTAGCCA
ACATAATTTTCGAGTGACTGAACTCAACTACCTCAGCGACTATGCGCTAGAACATTACGATAAAGATGACTTTCACGCTG
TTTCACTAAACCACCCTGTCTCATTCATTTTTGATGACATGGGTAAGCCTGGATGGTTAAAGTTTCTAGACGATATTATG
CCTAGTGGCGCTAGTAGACGATACTGGGTCAAACACCTAGATATTGAAGATCTTAGTTCTGATGAACAAGACTATGTTTT
GCTCAAATTTGGCACGATGTCGCCTATTGGTAACTTAAGAATAAAAGACTCCCTTCCTGAACGCTACGAAGTCGCAGACA
ACCTCTACTTCTCTGTTGATGATGTTAAAAATCGAGCAGGTGATTTCCTCGACTATGCACAGCAAAGAGGGGCAGCCGCA
GGTGGTGCAACAGGGGCTGGAGGTGAAGCGCCAAAACTGATCCTCCGATGTGGTTTCGATCATGGATCTGGCAGCGAAAA
AATATGGATTGATCCATACCAAGATGACAATAGCAGCCACGACTTACATTACTTAGTAAAGTACCCTAGAGGCTCAAGAA
GCACCATCGACTGTAATATTCTAAGAGCTGAGTTCTACTTCTACCACGAGCTAACTGAAATGGGAGTTGAAACGATCTCC
ACCGACGGTATGCGCTTGGAGGAAGGATTAAACTACCCTTCCTTATGGCTTCCTCGATTTGATGTTCAAATTATTGATCA
ACAAATAGAGCGATTTGGCATGGAATCAGTGTATTCCATTTTGAACAAAGGCGCTGGAGTCACTCTTGATCACGAAACAA
CAATCCGAACTCTCATAGAAAAAATTACTGAAAGTAATATGGTTAAACACCAAGGGTATAGGTTTGATACCCAAGCTTTC
GTTATTGAATGGGTGAAGAGAGATCTACTCAACATCTTGTTTGGTAATAGTGATAACCATGGCAGAAATACTTCATTCTT
AAAGGGTGATGGAGTCATAAAGCTTGCTCCCATTTACGATTTCGCACCAATGAAAGCCGATCCTGCAGGAATTCCAAGAA
CAACAAAATGGAAAGCCCCACTAGAGGTTGGCGGAACTTACGACTTTGTCGGCATTGCAGACACCTTATCTGATCTGGTG
CCAAAGGAAATGTTATTAAAAGAGCTAGCAATTACTGCAAGTAAATGCGTAGGCCTAAAGCAGAGACTCGCTTTACGAGG
AGTACCAGAACAAATCTTGGAGATGCCGGCCGTTGGACTTAATTATTTATCAGAAAAGCTCACTAAATGGGGGCTACTAT
GA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT58281 WP_000804849.1 NZ_CP013307:c445059-444592 [Vibrio cholerae]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT58281 NZ_CP013307:c445059-444592 [Vibrio cholerae]
ATGAAGCCAAGCAATAAAGAGTCCGTACAAGATACGGTAGCTCGTCTAAGAGAAAACCGAAATCAACAGCAGTCCAAGGT
TGGGAAGAATGCAACTCATTCGAGCAAAGAGCAAACACCACTCGATACTCCAACGAGATCGAAAGCCTCAAAAAACGCCA
AAGCAGTAAGTGCAGCAGAGCGCAAAACCGAGGCAAACAAAATCATCAAACACTTATTGCTTGGAGAGCTAACACAGGGC
CAAGCGCTGAAAAGTCTACGCATTAATATACTGGGACTCAAACAGGATGTATTCGCAAGACTTGTCGATGTCTCAAGAAA
AACACTCTCAGACATAGAGAATGATCGGGGCAGCTACAATACAGAGATTCTCAACAAGGTATTCAAACCATTTAGCTTAA
AAATCGGGCTGCTGCCTTCTTCCCCCGATGCGTTAAAATCGCTCTTAATGGATGGTGAGGATGGGTAA

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8