Detailed information of TA system TA058247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 391206..391734
• Replicon: chromosome
• Accession: NZ_CP013304
• Organism: Vibrio cholerae strain CRC711

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: ASZ82_RS16365
• Protein ID: WP_000221352.1
• Coordinates: 391206..391496 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: ASZ82_RS16370
• Protein ID: WP_000213183.1
• Coordinates: 391486..391734 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ82_RS16320	386966..387421	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ82_RS20510	387478..387573	+	96	WP_080368267.1	acetyltransferase	-
ASZ82_RS16325	387605..388018	+	414	WP_000049417.1	VOC family protein	-
ASZ82_RS16335	388169..388603	+	435	WP_000453957.1	GNAT family N-acetyltransferase	-
ASZ82_RS16350	389389..389559	+	171	WP_000483445.1	DUF645 family protein	-
ASZ82_RS16355	389787..390407	+	621	WP_000466864.1	YrdB family protein	-
ASZ82_RS16360	390555..391070	+	516	WP_001201528.1	lipocalin family protein	-
ASZ82_RS16365	391206..391496	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ82_RS16370	391486..391734	-	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ASZ82_RS16380	392305..392958	+	654	WP_000036957.1	site-specific DNA-methyltransferase	-
ASZ82_RS16385	393230..393655	+	426	WP_000415748.1	hypothetical protein	-
ASZ82_RS16390	393863..394258	+	396	WP_000649527.1	DUF3465 domain-containing protein	-
ASZ82_RS16395	394409..394939	+	531	WP_000415563.1	hypothetical protein	-
ASZ82_RS16400	395077..395577	+	501	WP_000789723.1	hypothetical protein	-
ASZ82_RS16405	395733..396131	+	399	WP_001882293.1	hypothetical protein	-
ASZ82_RS16410	396231..396695	-	465	WP_000140244.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304736..427674	122938
	-	inside	Integron	-	-	309816..427298	117482

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T58247 WP_000221352.1 NZ_CP013304:c391496-391206 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T58247 NZ_CP013304:c391496-391206 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT58247 WP_000213183.1 NZ_CP013304:c391734-391486 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT58247 NZ_CP013304:c391734-391486 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8