Detailed information of TA system TA058244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 367882..368420
• Replicon: chromosome
• Accession: NZ_CP013304
• Organism: Vibrio cholerae strain CRC711

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q9KMJ0
• Locus tag: ASZ82_RS16130
• Protein ID: WP_000802136.1
• Coordinates: 367882..368181 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P58093
• Locus tag: ASZ82_RS16135
• Protein ID: WP_001107719.1
• Coordinates: 368178..368420 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ82_RS16085	363817..363999	+	183	WP_000923182.1	DUF645 family protein	-
ASZ82_RS16090	364199..364672	+	474	WP_001161076.1	GrpB family protein	-
ASZ82_RS16095	364687..364779	+	93	WP_014378730.1	DUF3265 domain-containing protein	-
ASZ82_RS16100	364821..365447	+	627	WP_000365424.1	LysE family translocator	-
ASZ82_RS16105	365570..366268	+	699	WP_001890502.1	hypothetical protein	-
ASZ82_RS16110	366278..366388	+	111	WP_079857360.1	DUF3265 domain-containing protein	-
ASZ82_RS16120	366966..367145	+	180	WP_001883058.1	DUF645 family protein	-
ASZ82_RS16125	367359..367754	+	396	WP_000870857.1	DUF3465 domain-containing protein	-
ASZ82_RS16130	367882..368181	-	300	WP_000802136.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ82_RS16135	368178..368420	-	243	WP_001107719.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
ASZ82_RS16140	368649..369227	+	579	WP_000110120.1	hypothetical protein	-
ASZ82_RS16145	369249..369341	+	93	WP_014164112.1	DUF3265 domain-containing protein	-
ASZ82_RS16155	369765..370448	+	684	WP_000877436.1	hypothetical protein	-
ASZ82_RS16160	370662..370928	+	267	WP_000937852.1	hypothetical protein	-
ASZ82_RS16165	371083..371682	+	600	WP_000429495.1	hypothetical protein	-
ASZ82_RS16170	371963..372898	+	936	WP_000051985.1	restriction endonuclease	-
ASZ82_RS16175	372864..373004	+	141	WP_001883049.1	DUF3265 domain-containing protein	-
ASZ82_RS16180	373014..373409	+	396	WP_000046952.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304736..427674	122938
	-	inside	Integron	-	-	309816..427298	117482

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11589.29 Da
• Isoelectric Point: 9.9232

>T58244 WP_000802136.1 NZ_CP013304:c368181-367882 [Vibrio cholerae]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T58244 NZ_CP013304:c368181-367882 [Vibrio cholerae]
ATGAAACCATTTAATCTTACCGTCGCCGCCAAAGCCGATTTACGTGATATTGCTTTATTCACTCAACGACGCTGGGGAAA
AGAGCAGCGAAATGTTTATTTAAAGCAATTCGATGATTCCTTTTGGCTTTTAGCGGAAAATCCCGACATTGGTAAATCAT
GCGATGAAATCCGAGAGGGATACAGAAAATTTCCCCAAGGGAGTCACGTCATCTTTTATCAGCAAACCGGCAGCCAACAA
ATCAGGGTGATCCGAATTCTTCATAAGAGCATGGATGTGAACCCAATATTCGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8964.84 Da
• Isoelectric Point: 4.1677

>AT58244 WP_001107719.1 NZ_CP013304:c368420-368178 [Vibrio cholerae]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLIEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT58244 NZ_CP013304:c368420-368178 [Vibrio cholerae]
ATGGCTAAAAATACAAGTATCACTCTTGGTGAACACTTCGATGGCTTTATTACAAGCCAAATACAAAGTGGGCGTTACGG
CTCAGCAAGTGAAGTCATTCGCTCTGCGCTACGTCTACTCGAAAACCAAGAAACCAAACTACAGTCACTCCGTCAACTAC
TTATTGAAGGAGAGCAAAGTGGTGACGCTGATTATGACCTTGATAGCTTCATCAATGAACTCGATAGTGAAAACATTCGA
TGA

Structures

Toxin

• PDB: 7R5A

Antitoxin

• PDB: 7R5A
• PDB: 7B22
• AlphaFold DB: A0A151JFU4