Detailed information of TA system TA058242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 352205..352703
• Replicon: chromosome
• Accession: NZ_CP013304
• Organism: Vibrio cholerae strain CRC711

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: ASZ82_RS15960
• Protein ID: WP_000589156.1
• Coordinates: 352205..352471 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: ASZ82_RS15965
• Protein ID: WP_000643598.1
• Coordinates: 352458..352703 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ82_RS15920	347580..348140	+	561	WP_000400489.1	hypothetical protein	-
ASZ82_RS15925	348290..348964	+	675	WP_000073446.1	hypothetical protein	-
ASZ82_RS15930	349169..349411	+	243	WP_000107461.1	hypothetical protein	-
ASZ82_RS15935	349663..349941	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
ASZ82_RS20430	350316..350459	+	144	Protein_324	DUF645 family protein	-
ASZ82_RS20435	350513..350551	+	39	WP_082798268.1	hypothetical protein	-
ASZ82_RS15950	350763..351230	+	468	WP_001289288.1	OsmC family protein	-
ASZ82_RS15955	351358..352020	+	663	WP_000960654.1	hypothetical protein	-
ASZ82_RS15960	352205..352471	+	267	WP_000589156.1	BrnT family toxin	Toxin
ASZ82_RS15965	352458..352703	+	246	WP_000643598.1	hypothetical protein	Antitoxin
ASZ82_RS15970	352799..353593	+	795	WP_001911581.1	hypothetical protein	-
ASZ82_RS20440	353696..353824	+	129	WP_071908338.1	general secretion pathway protein GspI	-
ASZ82_RS15985	353885..354067	+	183	WP_000923182.1	DUF645 family protein	-
ASZ82_RS15990	354283..355287	+	1005	WP_000964920.1	LD-carboxypeptidase	-
ASZ82_RS15995	355440..355799	+	360	WP_001071541.1	VOC family protein	-
ASZ82_RS16000	356072..356305	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
ASZ82_RS16005	356573..357079	+	507	WP_000393074.1	hypothetical protein	-
ASZ82_RS16010	357236..357622	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304736..427674	122938
	-	inside	Integron	-	-	309816..427298	117482

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T58242 WP_000589156.1 NZ_CP013304:352205-352471 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T58242 NZ_CP013304:352205-352471 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT58242 WP_000643598.1 NZ_CP013304:352458-352703 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT58242 NZ_CP013304:352458-352703 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2