Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 237067..237709 Replicon chromosome
Accession NZ_CP013275
Organism Bacillus thuringiensis serovar israelensis strain AM65-52

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag ATN07_RS01350 Protein ID WP_000635965.1
Coordinates 237359..237709 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag ATN07_RS01345 Protein ID WP_000004570.1
Coordinates 237067..237354 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATN07_RS01320 232384..233346 + 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -
ATN07_RS01325 233339..233911 - 573 WP_000908528.1 rhomboid family intramembrane serine protease -
ATN07_RS01330 234005..234364 + 360 WP_000583420.1 holo-ACP synthase -
ATN07_RS01335 234521..235471 + 951 WP_003309006.1 outer membrane lipoprotein carrier protein LolA -
ATN07_RS01340 235589..236758 + 1170 WP_000390594.1 alanine racemase -
ATN07_RS01345 237067..237354 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
ATN07_RS01350 237359..237709 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ATN07_RS01355 237777..239945 + 2169 WP_000426211.1 RNA-binding transcriptional accessory protein -
ATN07_RS01360 240003..240119 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
ATN07_RS01365 240315..240773 + 459 WP_000343552.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T58206 WP_000635965.1 NZ_CP013275:237359-237709 [Bacillus thuringiensis serovar israelensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T58206 NZ_CP013275:237359-237709 [Bacillus thuringiensis serovar israelensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT58206 WP_000004570.1 NZ_CP013275:237067-237354 [Bacillus thuringiensis serovar israelensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT58206 NZ_CP013275:237067-237354 [Bacillus thuringiensis serovar israelensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References