Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 228966..229608 Replicon chromosome
Accession NZ_CP013274
Organism Bacillus thuringiensis strain CTC

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag ATN06_RS01495 Protein ID WP_000635963.1
Coordinates 229258..229608 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag ATN06_RS01490 Protein ID WP_000004570.1
Coordinates 228966..229253 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATN06_RS01465 224131..225093 + 963 WP_060629287.1 UV DNA damage repair endonuclease UvsE -
ATN06_RS01470 225260..225808 - 549 WP_060629288.1 rhomboid family intramembrane serine protease -
ATN06_RS01475 225900..226259 + 360 WP_060629289.1 holo-ACP synthase -
ATN06_RS01480 226416..227366 + 951 WP_060633080.1 outer membrane lipoprotein carrier protein LolA -
ATN06_RS01485 227485..228654 + 1170 WP_060629290.1 alanine racemase -
ATN06_RS01490 228966..229253 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
ATN06_RS01495 229258..229608 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ATN06_RS01500 229676..231844 + 2169 WP_060629291.1 RNA-binding transcriptional accessory protein -
ATN06_RS01505 231902..232018 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
ATN06_RS01510 232214..232672 + 459 WP_060629292.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T58204 WP_000635963.1 NZ_CP013274:229258-229608 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T58204 NZ_CP013274:229258-229608 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT58204 WP_000004570.1 NZ_CP013274:228966-229253 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT58204 NZ_CP013274:228966-229253 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References