Detailed information of TA system TA058203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 16340..16874
• Replicon: plasmid pDE4
• Accession: NZ_CP013268
• Organism: Sphingobium baderi strain DE-13

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W1S6F4
• Locus tag: ATN00_RS22230
• Protein ID: WP_004212931.1
• Coordinates: 16340..16621 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W1S0F5
• Locus tag: ATN00_RS22235
• Protein ID: WP_004212930.1
• Coordinates: 16608..16874 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATN00_RS22210	13956..14321	+	366	WP_004212937.1	hypothetical protein	-
ATN00_RS22215	14359..14754	+	396	WP_062069509.1	hypothetical protein	-
ATN00_RS22220	14751..15713	+	963	WP_004212934.1	tyrosine-type recombinase/integrase	-
ATN00_RS22225	15710..16330	-	621	WP_062069522.1	phosphoglycerate mutase family protein	-
ATN00_RS22230	16340..16621	-	282	WP_004212931.1	type II toxin-antitoxin system YafQ family toxin	Toxin
ATN00_RS22235	16608..16874	-	267	WP_004212930.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ATN00_RS22240	17278..17919	+	642	WP_062069512.1	hypothetical protein	-
ATN00_RS22245	17995..18648	+	654	WP_004212927.1	AAA family ATPase	-
ATN00_RS22250	18645..18959	+	315	WP_004212926.1	hypothetical protein	-
ATN00_RS22255	19608..20072	+	465	WP_031309919.1	GNAT family N-acetyltransferase	-
ATN00_RS22260	20162..21274	-	1113	WP_062069515.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..43676	43676

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-85)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10667.04 Da
• Isoelectric Point: 8.0689

>T58203 WP_004212931.1 NZ_CP013268:c16621-16340 [Sphingobium baderi]
MRTIDRSTRFKRDYKRESKGRHRATLDADLLPVLAALATDTPLDPKYRDHDLSGDWAGYRDCHVKPDLVLIYAKPDDETL
RLARLGSHSEVFG

Nucleotide

• Length: 282 bp

>T58203 NZ_CP013268:c16621-16340 [Sphingobium baderi]
ATGCGGACGATTGATCGTTCGACCCGGTTCAAGCGCGACTACAAGCGGGAATCAAAGGGCCGACATCGAGCGACCCTCGA
TGCCGATCTTCTGCCCGTCCTGGCCGCGCTCGCGACCGACACGCCGCTTGATCCCAAATACCGCGACCATGATCTGAGCG
GCGATTGGGCGGGCTACCGCGATTGCCACGTCAAGCCCGATCTGGTGCTGATCTACGCCAAGCCCGACGACGAAACGCTG
CGCCTCGCGCGCCTTGGCTCGCATAGCGAGGTATTCGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9448.88 Da
• Isoelectric Point: 5.8213

>AT58203 WP_004212930.1 NZ_CP013268:c16874-16608 [Sphingobium baderi]
MAVSDTYVRARIDNTTKERATAALGAMGLSISDAIRLLMLRIADERRLPFEVKVPNAATREAMAELAAGKGTKFASVDAL
MADLHADD

Nucleotide

• Length: 267 bp

>AT58203 NZ_CP013268:c16874-16608 [Sphingobium baderi]
ATGGCTGTTTCCGACACCTATGTCCGCGCCAGGATCGACAATACGACGAAGGAACGCGCCACCGCCGCGCTGGGCGCGAT
GGGCCTTTCGATCTCCGACGCTATCCGGCTGCTGATGCTGCGTATCGCCGACGAACGGCGCTTGCCTTTCGAGGTCAAGG
TTCCGAACGCCGCGACACGCGAGGCAATGGCAGAGCTGGCGGCGGGGAAGGGGACCAAGTTTGCCAGCGTCGATGCGCTG
ATGGCGGACCTCCATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: W1S6F4

Antitoxin

• AlphaFold DB: W1S0F5