Detailed information of TA system TA058201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 12738..13344
• Replicon: plasmid pDE3
• Accession: NZ_CP013267
• Organism: Sphingobium baderi strain DE-13

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0S3F6C3
• Locus tag: ATN00_RS21835
• Protein ID: WP_030091808.1
• Coordinates: 13036..13344 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ATN00_RS21830
• Protein ID: WP_030091807.1
• Coordinates: 12738..13043 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATN00_RS21795	8807..9235	+	429	WP_048575094.1	hypothetical protein	-
ATN00_RS21800	9251..10150	-	900	WP_053085604.1	cell wall hydrolase	-
ATN00_RS21805	10160..10690	-	531	WP_048575093.1	S26 family signal peptidase	-
ATN00_RS21810	10687..11016	-	330	WP_048575092.1	hypothetical protein	-
ATN00_RS21815	11013..11693	-	681	WP_048575091.1	AAA family ATPase	-
ATN00_RS21820	12094..12351	+	258	WP_048575090.1	antitoxin	-
ATN00_RS21825	12348..12674	+	327	WP_048575089.1	type II toxin-antitoxin system PemK/MazF family toxin	-
ATN00_RS21830	12738..13043	-	306	WP_030091807.1	putative addiction module antidote protein	Antitoxin
ATN00_RS21835	13036..13344	-	309	WP_030091808.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ATN00_RS21840	13454..14107	-	654	WP_048575088.1	hypothetical protein	-
ATN00_RS21845	14104..14937	-	834	WP_140404624.1	hypothetical protein	-
ATN00_RS21850	15595..15885	+	291	WP_062069476.1	hypothetical protein	-
ATN00_RS21855	15971..16297	-	327	WP_048575085.1	single-stranded DNA-binding protein	-
ATN00_RS21860	16586..17188	-	603	WP_048575084.1	recombinase family protein	-
ATN00_RS23820	17196..17432	-	237	WP_148564653.1	hypothetical protein	-
ATN00_RS21870	17627..17821	-	195	WP_156415414.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..73561	73561
	-	flank	IS/Tn	-	-	17911..18279	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-94)

Antitoxin

• Uncharacterized protein HI1420 (5-98)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11278.99 Da
• Isoelectric Point: 9.5709

>T58201 WP_030091808.1 NZ_CP013267:c13344-13036 [Sphingobium baderi]
MIELIKTSVFDAWLSGLRDRKAAWRVAARLDRLANGNPGDVKPVGGGLSEMRIDYGPGYRVYFMRRGPVLIVLLCGGDKS
SQARDIEEAKKLAALWKDGNDD

Nucleotide

• Length: 309 bp

>T58201 NZ_CP013267:c13344-13036 [Sphingobium baderi]
ATGATCGAGCTTATCAAAACGTCGGTGTTCGATGCTTGGCTTTCCGGTTTACGGGATCGCAAGGCGGCATGGCGCGTTGC
GGCTCGCCTGGATAGGCTCGCTAATGGAAACCCCGGCGACGTGAAGCCCGTGGGTGGCGGCCTCTCTGAAATGCGGATCG
ACTATGGCCCCGGCTATCGGGTCTACTTCATGCGCCGGGGACCGGTATTGATCGTGTTGCTCTGTGGCGGCGACAAGTCC
TCGCAGGCCCGCGACATTGAAGAAGCGAAGAAACTAGCCGCACTATGGAAGGACGGAAACGATGACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10942.31 Da
• Isoelectric Point: 4.8232

>AT58201 WP_030091807.1 NZ_CP013267:c13043-12738 [Sphingobium baderi]
MTDVTFSRYDSADYLKTEEDIAGYLEAVMEADGDNPAYVTRALGVVARARNISKLARDTGMSREGIYKALSEDGNPSFAT
VAKIAHALGLRIAFEPVRPTA

Nucleotide

• Length: 306 bp

>AT58201 NZ_CP013267:c13043-12738 [Sphingobium baderi]
ATGACTGACGTAACTTTCAGCCGGTATGACAGCGCCGACTATCTCAAAACCGAGGAAGATATTGCGGGCTATCTGGAAGC
TGTCATGGAGGCAGACGGCGACAATCCGGCCTATGTCACACGCGCCCTTGGCGTGGTTGCCCGCGCGCGGAATATTAGCA
AGCTGGCGCGCGACACCGGCATGAGCCGGGAAGGCATCTACAAGGCGCTTTCCGAGGACGGGAATCCCAGCTTTGCCACC
GTGGCGAAGATCGCGCACGCGCTGGGATTGCGGATCGCCTTTGAGCCGGTTCGGCCTACGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0S3F6C3