58200

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 12094..12674 Replicon plasmid pDE3
Accession NZ_CP013267
Organism Sphingobium baderi strain DE-13

Toxin (Protein)


Gene name pemK Uniprot ID A0A0S3F651
Locus tag ATN00_RS21825 Protein ID WP_048575089.1
Coordinates 12348..12674 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0S3F672
Locus tag ATN00_RS21820 Protein ID WP_048575090.1
Coordinates 12094..12351 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATN00_RS21795 8807..9235 + 429 WP_048575094.1 hypothetical protein -
ATN00_RS21800 9251..10150 - 900 WP_053085604.1 cell wall hydrolase -
ATN00_RS21805 10160..10690 - 531 WP_048575093.1 S26 family signal peptidase -
ATN00_RS21810 10687..11016 - 330 WP_048575092.1 hypothetical protein -
ATN00_RS21815 11013..11693 - 681 WP_048575091.1 AAA family ATPase -
ATN00_RS21820 12094..12351 + 258 WP_048575090.1 antitoxin Antitoxin
ATN00_RS21825 12348..12674 + 327 WP_048575089.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ATN00_RS21830 12738..13043 - 306 WP_030091807.1 putative addiction module antidote protein -
ATN00_RS21835 13036..13344 - 309 WP_030091808.1 type II toxin-antitoxin system RelE/ParE family toxin -
ATN00_RS21840 13454..14107 - 654 WP_048575088.1 hypothetical protein -
ATN00_RS21845 14104..14937 - 834 WP_140404624.1 hypothetical protein -
ATN00_RS21850 15595..15885 + 291 WP_062069476.1 hypothetical protein -
ATN00_RS21855 15971..16297 - 327 WP_048575085.1 single-stranded DNA-binding protein -
ATN00_RS21860 16586..17188 - 603 WP_048575084.1 recombinase family protein -
ATN00_RS23820 17196..17432 - 237 WP_148564653.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..73561 73561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-102)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11938.73 Da        Isoelectric Point: 8.0494

>T58200 WP_048575089.1 NZ_CP013267:12348-12674 [Sphingobium baderi]
MKRGDIYMVDLEPTHGREQRGHRPVVVVSPDDFNRTTGLPVIVPITNGGDFARRIGFAVPLSGTKTTGVVRCDQPRVLDL
DARNGRKIETLPQAIMDEVLARAVTIFE

Download         Length: 327 bp

>T58200 NZ_CP013267:12348-12674 [Sphingobium baderi]
ATGAAGCGCGGCGACATTTACATGGTCGATCTCGAACCCACGCATGGCCGCGAGCAACGCGGGCACCGTCCGGTTGTCGT
CGTCTCCCCTGACGACTTCAACCGGACTACCGGCTTGCCGGTTATAGTGCCGATTACGAATGGCGGCGACTTCGCGCGCC
GCATTGGATTCGCCGTGCCGCTCTCCGGCACAAAGACAACGGGCGTCGTGCGGTGCGATCAACCCCGCGTCCTCGATCTT
GACGCACGCAACGGGCGGAAGATCGAAACCTTGCCGCAAGCCATCATGGATGAAGTCTTGGCGCGAGCGGTGACGATCTT
CGAATAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9122.55 Da        Isoelectric Point: 4.9731

>AT58200 WP_048575090.1 NZ_CP013267:12094-12351 [Sphingobium baderi]
MHTTNLRKVGGSVMLVVPPALLDVLHLSAGASVGLAVDNGKLVVEPSTRPRYTMAELLAASDYSQPMPPEEREWVDTPAV
GRELI

Download         Length: 258 bp

>AT58200 NZ_CP013267:12094-12351 [Sphingobium baderi]
ATGCACACTACAAATCTTCGTAAGGTCGGGGGGTCGGTCATGCTGGTCGTTCCCCCTGCCCTGCTCGACGTTCTGCATCT
GTCCGCTGGCGCAAGCGTCGGTTTGGCCGTGGACAACGGCAAGCTGGTGGTCGAGCCGTCCACGCGGCCCCGCTACACCA
TGGCGGAGCTGCTGGCCGCGTCCGACTATTCGCAGCCTATGCCCCCCGAAGAACGGGAATGGGTTGATACCCCTGCGGTG
GGCCGCGAACTGATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S3F651

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3F672

References