Detailed information of TA system TA058197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 226721..227342
• Replicon: chromosome
• Accession: NZ_CP013264
• Organism: Sphingobium baderi strain DE-13

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A0S3EUN3
• Locus tag: ATN00_RS01175
• Protein ID: WP_062061074.1
• Coordinates: 226721..227029 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0S3EUM5
• Locus tag: ATN00_RS01180
• Protein ID: WP_062061077.1
• Coordinates: 227010..227342 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATN00_RS01135	222777..223775	-	999	WP_156415190.1	tyrosine-type recombinase/integrase	-
ATN00_RS01140	223736..224107	-	372	WP_030092871.1	hypothetical protein	-
ATN00_RS01145	224141..224506	-	366	WP_030092870.1	hypothetical protein	-
ATN00_RS01150	224503..224835	-	333	WP_030092869.1	hypothetical protein	-
ATN00_RS01155	224866..225096	-	231	WP_030092868.1	BrnA antitoxin family protein	-
ATN00_RS22695	225083..225356	-	274	Protein_226	BrnT family toxin	-
ATN00_RS01165	225496..226065	-	570	WP_062061072.1	PIN domain-containing protein	-
ATN00_RS01170	226062..226526	-	465	WP_030092865.1	helix-turn-helix domain-containing protein	-
ATN00_RS01175	226721..227029	+	309	WP_062061074.1	BrnT family toxin	Toxin
ATN00_RS01180	227010..227342	+	333	WP_062061077.1	BrnA antitoxin family protein	Antitoxin
ATN00_RS01185	227346..227603	+	258	Protein_231	DUF2442 domain-containing protein	-
ATN00_RS01190	227632..228351	-	720	WP_062061080.1	hypothetical protein	-
ATN00_RS01195	228348..228782	-	435	WP_062061082.1	putative toxin-antitoxin system toxin component, PIN family	-
ATN00_RS01200	228786..229031	-	246	WP_197413644.1	ribbon-helix-helix domain-containing protein	-
ATN00_RS01205	229094..232255	-	3162	WP_062061085.1	Ti-type conjugative transfer relaxase TraA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	tufA	112541..293149	180608

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (8-93)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (17-99)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11782.17 Da
• Isoelectric Point: 5.6397

>T58197 WP_062061074.1 NZ_CP013264:226721-227029 [Sphingobium baderi]
MSSVRFSWNPLKAEANLKKHGVSFETAARAFADPFALSEQDRIEGGEQRWQTLGLVEGHLLLLMAHTVAEDDDDGEYVEV
IHIISARKADRKERRRYEEERR

Nucleotide

• Length: 309 bp

>T58197 NZ_CP013264:226721-227029 [Sphingobium baderi]
ATGTCGTCCGTTCGCTTTTCGTGGAACCCGCTCAAAGCGGAAGCCAATCTGAAAAAGCACGGCGTCAGTTTCGAGACAGC
GGCCCGCGCCTTCGCGGACCCTTTCGCACTCAGTGAGCAAGATCGGATCGAAGGCGGCGAGCAGCGATGGCAGACCTTGG
GCCTGGTGGAAGGACATTTGCTCCTGCTGATGGCCCACACCGTCGCCGAGGATGACGATGACGGCGAATATGTGGAGGTC
ATTCATATCATTTCGGCCCGAAAAGCCGATCGGAAGGAAAGGCGACGTTATGAAGAAGAAAGACGCTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12465.21 Da
• Isoelectric Point: 8.4293

>AT58197 WP_062061077.1 NZ_CP013264:227010-227342 [Sphingobium baderi]
MKKKDADTVRFQLDPADLPPLTDAQKAELDALQALPDSEIDYSDVPALSDDFWKGAERGRFYKPIKQQVTARLDADVLAW
LKSQGKGYQARMNAILRREMLATVERRKSA

Nucleotide

• Length: 333 bp

>AT58197 NZ_CP013264:227010-227342 [Sphingobium baderi]
ATGAAGAAGAAAGACGCTGATACCGTGCGCTTCCAGCTCGATCCGGCGGACCTGCCCCCGCTGACCGACGCGCAGAAGGC
CGAACTAGACGCCTTGCAGGCGCTGCCGGATAGCGAAATCGACTATAGCGACGTGCCGGCGCTATCGGACGATTTCTGGA
AGGGTGCCGAGCGCGGCCGCTTCTACAAGCCGATCAAGCAACAGGTCACGGCGCGGCTCGATGCTGATGTGCTGGCGTGG
CTCAAATCGCAAGGCAAGGGCTACCAAGCCCGCATGAACGCTATCCTGCGGCGCGAAATGCTGGCGACCGTGGAGCGGCG
CAAAAGCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0S3EUN3

Antitoxin

• AlphaFold DB: A0A0S3EUM5