Detailed information of TA system TA058194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 24461..25029
• Replicon: plasmid unnamed
• Accession: NZ_CP013250
• Organism: Vibrio parahaemolyticus strain FORC_022

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1Y1BA95
• Locus tag: FORC22_RS24925
• Protein ID: WP_023624650.1
• Coordinates: 24461..24802 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FORC22_RS24930
• Protein ID: WP_071653122.1
• Coordinates: 24799..25029 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC22_RS24895	19865..20053	-	189	WP_140062600.1	hypothetical protein	-
FORC22_RS24900	20533..21609	+	1077	WP_071653120.1	replication initiation protein	-
FORC22_RS27235	22501..22592	+	92	Protein_21	DUF3265 domain-containing protein	-
FORC22_RS24910	22853..23584	+	732	WP_071653121.1	AHH domain-containing protein	-
FORC22_RS24915	23584..24158	+	575	Protein_23	hypothetical protein	-
FORC22_RS24925	24461..24802	-	342	WP_023624650.1	endoribonuclease MazF	Toxin
FORC22_RS24930	24799..25029	-	231	WP_071653122.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FORC22_RS24935	25480..26205	+	726	WP_017449237.1	site-specific integrase	-
FORC22_RS24940	26200..26703	-	504	Protein_27	recombinase family protein	-
FORC22_RS24945	27897..28418	+	522	WP_071653123.1	hypothetical protein	-
FORC22_RS24950	28430..29335	+	906	WP_071653124.1	endonuclease/exonuclease/phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..101545	101545

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-107)

Antitoxin

• Antitoxin PemI-like (1-75)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12159.65 Da
• Isoelectric Point: 5.8124

>T58194 WP_023624650.1 NZ_CP013250:c24802-24461 [Vibrio parahaemolyticus]
MTYAVPEKGDVVVMSFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Nucleotide

• Length: 342 bp

>T58194 NZ_CP013250:c24802-24461 [Vibrio parahaemolyticus]
ATGACGTATGCAGTACCAGAGAAAGGCGATGTAGTAGTAATGAGTTTTAATCCGCAAAGCGGACACGAACAAGCGGGAGA
CAGTCGGCCTGGGCTGGTCATTTCCCCGAAAGAGTTTAACCAAGCAACAGGCTTTGCTCTGGTATGCCCCATAACCAACC
AGTCAAAAGGGTATCCGTTTGAAGTTGAAATAACCGGAAGCCAAAAAACAACGGGTGTGGTGCTGGCTGACCAAGTGAAA
TCCTTGGATTGGCGCGCGCGTCGCGCTCGTGTGGTGGACTCCGTTTCAAACGAAACACTGGGGACTGTGCGGGATTATGT
CACAGCGATTGCTAATGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8472.84 Da
• Isoelectric Point: 6.4604

>AT58194 WP_071653122.1 NZ_CP013250:c25029-24799 [Vibrio parahaemolyticus]
MSQTIAKWGHSAAVRIPSKVLKSMDMAVGDKVEVKVFNRQIIIEPAKPTLAQLLSQITDENRHDEVITETMGNELL

Nucleotide

• Length: 231 bp

>AT58194 NZ_CP013250:c25029-24799 [Vibrio parahaemolyticus]
ATGTCACAAACAATAGCTAAGTGGGGCCACAGCGCAGCTGTGCGTATACCTTCTAAGGTATTGAAAAGTATGGATATGGC
GGTGGGTGACAAAGTGGAGGTTAAAGTGTTCAATCGACAAATTATCATTGAACCTGCAAAACCAACACTCGCCCAGCTTT
TGAGTCAGATCACTGATGAAAACCGCCATGATGAAGTAATCACCGAAACTATGGGTAATGAATTGTTATGA

Structures

Toxin

• AlphaFold DB: A0A1Y1BA95