Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2168358..2168872 Replicon chromosome
Accession NZ_CP013218
Organism Staphylococcus aureus strain LA-MRSA ST398 isolate E154

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag AS852_RS11225 Protein ID WP_000581792.1
Coordinates 2168358..2168567 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag AS852_RS11230 Protein ID WP_000138932.1
Coordinates 2168579..2168872 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AS852_RS11205 2163869..2165224 - 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
AS852_RS11210 2165239..2166309 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
AS852_RS11215 2166627..2167829 + 1203 WP_001109939.1 rod shape-determining protein RodA -
AS852_RS11220 2168093..2168230 - 138 WP_000828354.1 hypothetical protein -
AS852_RS11225 2168358..2168567 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
AS852_RS11230 2168579..2168872 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
AS852_RS11235 2169038..2170522 + 1485 WP_000571550.1 cardiolipin synthase -
AS852_RS11240 2170550..2171197 + 648 WP_001187614.1 HD domain-containing protein -
AS852_RS11250 2171477..2171797 + 321 WP_001825748.1 hypothetical protein -
AS852_RS11255 2171794..2173109 - 1316 Protein_2066 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T58127 WP_000581792.1 NZ_CP013218:c2168567-2168358 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T58127 NZ_CP013218:c2168567-2168358 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT58127 WP_000138932.1 NZ_CP013218:c2168872-2168579 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT58127 NZ_CP013218:c2168872-2168579 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References