Detailed information of TA system TA058126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2148621..2149150
• Replicon: chromosome
• Accession: NZ_CP013218
• Organism: Staphylococcus aureus strain LA-MRSA ST398 isolate E154

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AS852_RS11135
• Protein ID: WP_000621175.1
• Coordinates: 2148621..2148983 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AS852_RS11140
• Protein ID: WP_000948331.1
• Coordinates: 2148980..2149150 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS852_RS11110	2145599..2146369	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
AS852_RS11115	2146344..2146823	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
AS852_RS11120	2146825..2147151	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AS852_RS11125	2147270..2148271	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AS852_RS11135	2148621..2148983	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AS852_RS11140	2148980..2149150	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AS852_RS11145	2149235..2150383	-	1149	WP_001281139.1	alanine racemase	-
AS852_RS11150	2150449..2150808	-	360	WP_000581197.1	holo-ACP synthase	-
AS852_RS11155	2150812..2151303	-	492	WP_001286980.1	PH domain-containing protein	-
AS852_RS11160	2151296..2152873	-	1578	WP_001294650.1	PH domain-containing protein	-
AS852_RS11165	2152866..2153345	-	480	WP_001287081.1	hypothetical protein	-
AS852_RS11170	2153554..2154114	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T58126 WP_000621175.1 NZ_CP013218:c2148983-2148621 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T58126 NZ_CP013218:c2148983-2148621 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT58126 WP_000948331.1 NZ_CP013218:c2149150-2148980 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT58126 NZ_CP013218:c2149150-2148980 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1