Detailed information of TA system TA058117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1115060..1115668
• Replicon: chromosome
• Accession: NZ_CP013203
• Organism: Paenibacillus sp. IHB B 3084

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: ASL14_RS05245
• Protein ID: WP_007429335.1
• Coordinates: 1115060..1115410 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ASL14_RS05250
• Protein ID: WP_170970806.1
• Coordinates: 1115417..1115668 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASL14_RS05215	1110568..1111440	+	873	WP_134910711.1	Cof-type HAD-IIB family hydrolase	-
ASL14_RS05230	1111746..1112258	-	513	WP_134910712.1	SprT family protein	-
ASL14_RS05235	1112419..1112553	+	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
ASL14_RS05240	1112657..1114879	-	2223	WP_189338230.1	RNA-binding transcriptional accessory protein	-
ASL14_RS05245	1115060..1115410	-	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ASL14_RS05250	1115417..1115668	-	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
ASL14_RS05255	1115993..1117189	-	1197	WP_134910714.1	alanine racemase	-
ASL14_RS05260	1117452..1118774	-	1323	WP_134910715.1	outer membrane lipoprotein carrier protein LolA	-
ASL14_RS05265	1119175..1120047	-	873	WP_134914403.1	class II fructose-1,6-bisphosphate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T58117 WP_007429335.1 NZ_CP013203:c1115410-1115060 [Paenibacillus sp. IHB B 3084]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T58117 NZ_CP013203:c1115410-1115060 [Paenibacillus sp. IHB B 3084]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTCAGTCCCACCGTGATCGTGGCAGCCATCACCGCCCAGATTCAGAAGG
CGAAGCTGCCCACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGCGATTCTGTCGTTCTTCTGGAGCAAATT
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAAATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT58117 WP_170970806.1 NZ_CP013203:c1115668-1115417 [Paenibacillus sp. IHB B 3084]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT58117 NZ_CP013203:c1115668-1115417 [Paenibacillus sp. IHB B 3084]
ATGATCAGTTTGCCGGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCCATGAAGCTGTATGTAGGCGAACGAAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGTTATATGGAGA
TGGCTAAAATTAACTTGACCATGGCCTCCGAAGCCTTTCATGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1