Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 12324..12849 Replicon plasmid pFORC31.4
Accession NZ_CP013194
Organism Escherichia coli strain FORC_031

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FORC31_RS25555 Protein ID WP_001159871.1
Coordinates 12324..12629 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FORC31_RS25560 Protein ID WP_000813634.1
Coordinates 12631..12849 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC31_RS25530 8568..9271 - 704 Protein_7 helix-turn-helix domain-containing protein -
FORC31_RS25535 9519..10199 + 681 WP_000452091.1 EAL domain-containing protein -
FORC31_RS25540 10639..11280 - 642 WP_000470631.1 recombinase family protein -
FORC31_RS25550 11610..12323 - 714 WP_000016957.1 site-specific integrase -
FORC31_RS25555 12324..12629 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FORC31_RS25560 12631..12849 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FORC31_RS25565 13294..13551 + 258 WP_000343092.1 hypothetical protein -
FORC31_RS25570 13551..14081 + 531 WP_011387704.1 hypothetical protein -
FORC31_RS25575 14325..15167 + 843 WP_160371899.1 polysaccharide deacetylase family protein -
FORC31_RS25580 15170..16258 + 1089 WP_004100285.1 putative hexosyltransferase CapU -
FORC31_RS25585 16263..17213 + 951 WP_001699589.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..39792 39792
- flank IS/Tn - - 10639..11262 623


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T58113 WP_001159871.1 NZ_CP013194:c12629-12324 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T58113 NZ_CP013194:c12629-12324 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT58113 WP_000813634.1 NZ_CP013194:c12849-12631 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT58113 NZ_CP013194:c12849-12631 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References