Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21849..22492 Replicon plasmid pFORC31.3
Accession NZ_CP013193
Organism Escherichia coli strain FORC_031

Toxin (Protein)


Gene name vagD Uniprot ID Q2VNX7
Locus tag FORC31_RS25225 Protein ID WP_001044769.1
Coordinates 22076..22492 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag FORC31_RS25220 Protein ID WP_001261287.1
Coordinates 21849..22079 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC31_RS25190 16873..17265 - 393 WP_000272015.1 plasmid partitioning/stability family protein -
FORC31_RS25195 17269..18243 - 975 WP_001217833.1 hypothetical protein -
FORC31_RS29000 18482..18856 - 375 WP_000752652.1 hypothetical protein -
FORC31_RS25205 18856..19488 - 633 WP_000312331.1 ParA family protein -
FORC31_RS25210 20090..20875 - 786 WP_001164207.1 site-specific integrase -
FORC31_RS25215 20877..21290 - 414 WP_000465043.1 hypothetical protein -
FORC31_RS25220 21849..22079 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FORC31_RS25225 22076..22492 + 417 WP_001044769.1 type II toxin-antitoxin system VapC family toxin Toxin
FORC31_RS25230 22654..24792 - 2139 WP_000350636.1 AAA family ATPase -
FORC31_RS25240 25395..25985 + 591 WP_012000738.1 hypothetical protein -
FORC31_RS25245 26003..26350 - 348 WP_000142434.1 hypothetical protein -
FORC31_RS25250 26469..26852 + 384 WP_000762576.1 hypothetical protein -
FORC31_RS27990 26800..27018 - 219 Protein_34 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - csbD / csdC / cooA / csbB 1..66990 66990


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15183.63 Da        Isoelectric Point: 7.7805

>T58112 WP_001044769.1 NZ_CP013193:22076-22492 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKVKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T58112 NZ_CP013193:22076-22492 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGGTTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT58112 WP_001261287.1 NZ_CP013193:21849-22079 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT58112 NZ_CP013193:21849-22079 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZGW5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References