Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 90166..90691 Replicon plasmid pFORC31.1
Accession NZ_CP013191
Organism Escherichia coli strain FORC_031

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FORC31_RS24640 Protein ID WP_001159871.1
Coordinates 90386..90691 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID D7GK94
Locus tag FORC31_RS24635 Protein ID WP_000829078.1
Coordinates 90166..90384 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC31_RS24610 85713..86663 - 951 WP_001398306.1 virulence factor VirK -
FORC31_RS27780 86668..87756 - 1089 WP_012131088.1 putative hexosyltransferase CapU -
FORC31_RS24620 87759..88601 - 843 WP_162749921.1 polysaccharide deacetylase family protein -
FORC31_RS24625 88845..89387 - 543 WP_000194544.1 hypothetical protein -
FORC31_RS24630 89387..89644 - 258 WP_000343095.1 hypothetical protein -
FORC31_RS24635 90166..90384 + 219 WP_000829078.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FORC31_RS24640 90386..90691 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FORC31_RS24645 90692..91498 + 807 WP_000016960.1 site-specific integrase -
FORC31_RS24650 91676..92320 + 645 WP_001144037.1 ParA family protein -
FORC31_RS24655 92407..92715 + 309 WP_000030204.1 molecular chaperone GroEL -
FORC31_RS28975 92925..94155 + 1231 Protein_112 IS66 family transposase -
FORC31_RS24675 94408..95537 - 1130 Protein_113 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - etpB / etpA / eltA / eltB / cs3 1..106519 106519


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T58111 WP_001159871.1 NZ_CP013191:90386-90691 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T58111 NZ_CP013191:90386-90691 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8440.51 Da        Isoelectric Point: 6.3212

>AT58111 WP_000829078.1 NZ_CP013191:90166-90384 [Escherichia coli]
MKRRITVTVDSDSYQLLKAYDVNISGLVSTIMQNEARRLRAERWKAENREGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT58111 NZ_CP013191:90166-90384 [Escherichia coli]
ATGAAACGGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCATCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCGGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A7ZGP5

References