Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4363842..4364339 Replicon chromosome
Accession NZ_CP013190
Organism Escherichia coli strain FORC_031

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FORC31_RS21820 Protein ID WP_001173343.1
Coordinates 4363842..4364015 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag FORC31_RS21825 Protein ID WP_071597404.1
Coordinates 4364043..4364339 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC31_RS21810 4360507..4361964 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
FORC31_RS21815 4362201..4363718 + 1518 WP_001295074.1 lysine--tRNA ligase -
FORC31_RS21820 4363842..4364015 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FORC31_RS21825 4364043..4364339 - 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FORC31_RS21835 4364566..4364838 - 273 WP_000405647.1 N-acetyltransferase -
FORC31_RS21840 4364850..4365080 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
FORC31_RS21845 4365261..4366892 + 1632 WP_001216490.1 sensor histidine kinase -
FORC31_RS21850 4366889..4367608 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4347796..4364339 16543


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T58110 WP_001173343.1 NZ_CP013190:c4364015-4363842 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T58110 NZ_CP013190:c4364015-4363842 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT58110 WP_071597404.1 NZ_CP013190:c4364339-4364043 [Escherichia coli]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT58110 NZ_CP013190:c4364339-4364043 [Escherichia coli]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References