Detailed information of TA system TA058108

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4138547..4139379
• Replicon: chromosome
• Accession: NZ_CP013190
• Organism: Escherichia coli strain FORC_031

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: FORC31_RS20610
• Protein ID: WP_000854753.1
• Coordinates: 4138547..4138921 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: FORC31_RS20615
• Protein ID: WP_001540478.1
• Coordinates: 4139011..4139379 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC31_RS28950	4136275..4136433	-	159	WP_001467148.1	hypothetical protein	-
FORC31_RS20590	4136533..4136709	-	177	WP_000839290.1	DUF957 domain-containing protein	-
FORC31_RS20595	4136726..4137088	-	363	Protein_3951	hypothetical protein	-
FORC31_RS20605	4137182..4138410	+	1229	WP_155105063.1	IS3 family transposase	-
FORC31_RS20610	4138547..4138921	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
FORC31_RS20615	4139011..4139379	-	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FORC31_RS20625	4139542..4139763	-	222	WP_000692311.1	DUF987 domain-containing protein	-
FORC31_RS20630	4139826..4140302	-	477	WP_001186786.1	RadC family protein	-
FORC31_RS20635	4140318..4140800	-	483	WP_001508976.1	antirestriction protein	-
FORC31_RS20640	4140892..4141710	-	819	WP_001508975.1	DUF945 domain-containing protein	-
FORC31_RS20645	4141865..4142023	-	159	WP_001323397.1	DUF905 family protein	-
FORC31_RS20650	4142128..4142553	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
FORC31_RS20655	4142550..4142900	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4119746..4164891	45145
	-	inside	IScluster/Tn	-	-	4137182..4144544	7362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T58108 WP_000854753.1 NZ_CP013190:c4138921-4138547 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T58108 NZ_CP013190:c4138921-4138547 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT58108 WP_001540478.1 NZ_CP013190:c4139379-4139011 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT58108 NZ_CP013190:c4139379-4139011 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68