Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2559646..2560284 Replicon chromosome
Accession NZ_CP013190
Organism Escherichia coli strain FORC_031

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FORC31_RS12740 Protein ID WP_000813794.1
Coordinates 2560108..2560284 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FORC31_RS12735 Protein ID WP_001270286.1
Coordinates 2559646..2560062 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC31_RS12715 2554798..2555739 - 942 WP_001251313.1 ABC transporter permease -
FORC31_RS12720 2555740..2556753 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
FORC31_RS12725 2556771..2557916 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FORC31_RS12730 2558161..2559567 - 1407 WP_000760586.1 PLP-dependent aminotransferase family protein -
FORC31_RS12735 2559646..2560062 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FORC31_RS12740 2560108..2560284 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FORC31_RS12745 2560506..2560736 + 231 WP_000494244.1 YncJ family protein -
FORC31_RS12750 2560828..2562789 - 1962 WP_001355676.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FORC31_RS12755 2562862..2563398 - 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
FORC31_RS12760 2563451..2564665 + 1215 WP_071597387.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2564705..2565970 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T58104 WP_000813794.1 NZ_CP013190:c2560284-2560108 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T58104 NZ_CP013190:c2560284-2560108 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT58104 WP_001270286.1 NZ_CP013190:c2560062-2559646 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT58104 NZ_CP013190:c2560062-2559646 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References