Detailed information of TA system TA058089

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 46737..47460
• Replicon: chromosome
• Accession: NZ_CP013190
• Organism: Escherichia coli strain FORC_031

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: FORC31_RS00255
• Protein ID: WP_000854735.1
• Coordinates: 46737..47114 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FORC31_RS00260
• Protein ID: WP_000186655.1
• Coordinates: 47161..47460 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC31_RS00225	42084..43007	-	924	WP_000535961.1	carboxylate/amino acid/amine transporter	-
FORC31_RS00230	43118..44302	-	1185	WP_001172882.1	sugar efflux transporter	-
FORC31_RS00240	45098..45940	-	843	WP_000036706.1	DUF4942 domain-containing protein	-
FORC31_RS00245	46037..46234	-	198	WP_000839266.1	DUF957 domain-containing protein	-
FORC31_RS00250	46252..46740	-	489	WP_000761680.1	hypothetical protein	-
FORC31_RS00255	46737..47114	-	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
FORC31_RS00260	47161..47460	-	300	WP_000186655.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FORC31_RS00265	47615..47836	-	222	WP_000692309.1	DUF987 domain-containing protein	-
FORC31_RS00270	47899..48375	-	477	WP_001366855.1	RadC family protein	-
FORC31_RS00275	48391..48870	-	480	WP_000844100.1	antirestriction protein	-
FORC31_RS00280	48952..49770	-	819	WP_001175149.1	DUF945 domain-containing protein	-
FORC31_RS00285	49860..50093	-	234	WP_001278287.1	DUF905 family protein	-
FORC31_RS00290	50099..50776	-	678	WP_001097301.1	hypothetical protein	-
FORC31_RS00295	50924..51604	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (1-92)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T58089 WP_000854735.1 NZ_CP013190:c47114-46737 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T58089 NZ_CP013190:c47114-46737 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10863.35 Da
• Isoelectric Point: 6.0452

>AT58089 WP_000186655.1 NZ_CP013190:c47460-47161 [Escherichia coli]
VTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEADTLG
SCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 300 bp

>AT58089 NZ_CP013190:c47460-47161 [Escherichia coli]
GTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGG
GCAGTTTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCA
GCGGTGAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGC
AGCTGTGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: U9XMP3