Detailed information of TA system TA058079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 67785..68344
• Replicon: plasmid FORC_029p
• Accession: NZ_CP013186
• Organism: Escherichia coli strain FORC_029

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G4VUF8
• Locus tag: FORC29_RS27665
• Protein ID: WP_000421273.1
• Coordinates: 67785..68060 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: FORC29_RS27670
• Protein ID: WP_001387467.1
• Coordinates: 68060..68344 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC29_RS27625	63835..64575	-	741	WP_001388582.1	tyrosine-type recombinase/integrase	-
FORC29_RS27630	64696..64878	-	183	WP_048657295.1	hypothetical protein	-
FORC29_RS27640	65222..65529	-	308	Protein_84	type II toxin-antitoxin system ParD family antitoxin	-
FORC29_RS27650	66022..67190	+	1169	Protein_85	hypothetical protein	-
FORC29_RS27655	67223..67351	+	129	Protein_86	IS481 family transposase	-
FORC29_RS27660	67386..67679	-	294	WP_001105065.1	hypothetical protein	-
FORC29_RS27665	67785..68060	-	276	WP_000421273.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC29_RS27670	68060..68344	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
FORC29_RS27685	68894..69514	-	621	Protein_90	replication initiation protein	-
FORC29_RS27690	69504..70037	-	534	WP_001505108.1	incFII RepA family protein	-
FORC29_RS27695	70030..70104	-	75	WP_001388587.1	RepA leader peptide Tap	-
FORC29_RS27705	70337..70594	-	258	WP_000083826.1	replication regulatory protein RepA	-
FORC29_RS27710	70834..71424	-	591	WP_000766804.1	DUF2726 domain-containing protein	-
FORC29_RS27715	71463..71672	-	210	Protein_95	hemolysin expression modulator Hha	-
FORC29_RS27720	71718..72179	-	462	WP_001233835.1	thermonuclease family protein	-
FORC29_RS27725	72424..72636	-	213	WP_001388588.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B	pic / aap/aspU / agg3A / agg3B / agg3C / agg3D	1..77339	77339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10765.45 Da
• Isoelectric Point: 9.9324

>T58079 WP_000421273.1 NZ_CP013186:c68060-67785 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T58079 NZ_CP013186:c68060-67785 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT58079 WP_001387467.1 NZ_CP013186:c68344-68060 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT58079 NZ_CP013186:c68344-68060 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6H1

Antitoxin

• AlphaFold DB: A0A0E0Y880