Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56810..57335 Replicon plasmid FORC_029p
Accession NZ_CP013186
Organism Escherichia coli strain FORC_029

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FORC29_RS27580 Protein ID WP_001159871.1
Coordinates 56810..57115 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FORC29_RS27585 Protein ID WP_000813634.1
Coordinates 57117..57335 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC29_RS27545 52009..52482 + 474 Protein_67 IS3 family transposase -
FORC29_RS27550 52493..53320 + 828 Protein_68 translesion error-prone DNA polymerase V subunit UmuC -
FORC29_RS27555 53352..53744 - 393 WP_000340832.1 plasmid partitioning/stability family protein -
FORC29_RS27560 53749..54720 - 972 WP_001103689.1 hypothetical protein -
FORC29_RS27565 54949..55593 + 645 WP_000633911.1 AAA family ATPase -
FORC29_RS27570 55587..55862 + 276 WP_000239529.1 hypothetical protein -
FORC29_RS27575 56000..56809 - 810 WP_000016979.1 site-specific integrase -
FORC29_RS27580 56810..57115 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FORC29_RS27585 57117..57335 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FORC29_RS27590 57697..57885 - 189 Protein_76 IS66 family transposase -
FORC29_RS27595 57946..59079 + 1134 WP_000555401.1 IS110-like element ISEc45 family transposase -
FORC29_RS27600 59333..60757 - 1425 Protein_78 IS66-like element ISEc23 family transposase -
FORC29_RS27605 60788..61138 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
FORC29_RS27610 61135..61560 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B pic / aap/aspU / agg3A / agg3B / agg3C / agg3D 1..77339 77339


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T58078 WP_001159871.1 NZ_CP013186:c57115-56810 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T58078 NZ_CP013186:c57115-56810 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT58078 WP_000813634.1 NZ_CP013186:c57335-57117 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT58078 NZ_CP013186:c57335-57117 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References