Detailed information of TA system TA058071

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4699387..4700188
• Replicon: chromosome
• Accession: NZ_CP013185
• Organism: Escherichia coli strain FORC_029

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D3GUT5
• Locus tag: FORC29_RS24770
• Protein ID: WP_001094430.1
• Coordinates: 4699811..4700188 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FORC29_RS24765
• Protein ID: WP_088888735.1
• Coordinates: 4699387..4699764 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC29_RS24730	4694590..4696098	-	1509	WP_001189118.1	group II intron reverse transcriptase/maturase	-
FORC29_RS24740	4696771..4697510	-	740	Protein_4506	IS3 family transposase	-
FORC29_RS28260	4697567..4697692	+	126	Protein_4507	DUF945 domain-containing protein	-
FORC29_RS24750	4698034..4698507	+	474	WP_000855057.1	antirestriction protein	-
FORC29_RS24755	4698523..4698999	+	477	WP_157787305.1	RadC family protein	-
FORC29_RS24760	4699086..4699307	+	222	WP_000692352.1	DUF987 domain-containing protein	-
FORC29_RS24765	4699387..4699764	+	378	WP_088888735.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FORC29_RS24770	4699811..4700188	+	378	WP_001094430.1	TA system toxin CbtA family protein	Toxin
FORC29_RS24775	4700185..4700673	+	489	WP_000761715.1	hypothetical protein	-
FORC29_RS24780	4700693..4700890	+	198	WP_000772029.1	DUF957 domain-containing protein	-
FORC29_RS24785	4700975..4701817	+	843	WP_001290187.1	DUF4942 domain-containing protein	-
FORC29_RS24795	4702566..4704104	+	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13971.82 Da
• Isoelectric Point: 6.8608

>T58071 WP_001094430.1 NZ_CP013185:4699811-4700188 [Escherichia coli]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T58071 NZ_CP013185:4699811-4700188 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13705.60 Da
• Isoelectric Point: 6.0568

>AT58071 WP_088888735.1 NZ_CP013185:4699387-4699764 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT58071 NZ_CP013185:4699387-4699764 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6M7