Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3876892..3877510 Replicon chromosome
Accession NZ_CP013185
Organism Escherichia coli strain FORC_029

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FORC29_RS20560 Protein ID WP_001291435.1
Coordinates 3877292..3877510 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FORC29_RS20555 Protein ID WP_000344800.1
Coordinates 3876892..3877266 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC29_RS20545 3871981..3873174 + 1194 WP_001386618.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FORC29_RS20550 3873197..3876346 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FORC29_RS20555 3876892..3877266 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FORC29_RS20560 3877292..3877510 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FORC29_RS20565 3877681..3878232 + 552 WP_000102564.1 maltose O-acetyltransferase -
FORC29_RS20570 3878348..3878818 + 471 WP_000136192.1 YlaC family protein -
FORC29_RS20575 3878982..3880532 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FORC29_RS20580 3880574..3880927 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FORC29_RS20590 3881306..3881617 + 312 WP_000409911.1 MGMT family protein -
FORC29_RS20595 3881648..3882220 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T58067 WP_001291435.1 NZ_CP013185:3877292-3877510 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T58067 NZ_CP013185:3877292-3877510 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT58067 WP_000344800.1 NZ_CP013185:3876892-3877266 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT58067 NZ_CP013185:3876892-3877266 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References