Detailed information of TA system TA058066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3291667..3292148
• Replicon: chromosome
• Accession: NZ_CP013185
• Organism: Escherichia coli strain FORC_029

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: FORC29_RS17555
• Protein ID: WP_033812095.1
• Coordinates: 3291858..3292148 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: -
• Locus tag: FORC29_RS17550
• Protein ID: WP_033812094.1
• Coordinates: 3291667..3291858 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC29_RS17500	3286812..3287138	-	327	WP_001369935.1	hypothetical protein	-
FORC29_RS17505	3287139..3287450	-	312	WP_001289674.1	hypothetical protein	-
FORC29_RS17510	3287437..3287742	-	306	WP_001376361.1	DUF4406 domain-containing protein	-
FORC29_RS17515	3287739..3288020	-	282	WP_087614068.1	DNA-binding protein	-
FORC29_RS17520	3288053..3288769	-	717	WP_088888717.1	DUF1627 domain-containing protein	-
FORC29_RS17525	3288803..3289345	-	543	WP_072130322.1	replication protein	-
FORC29_RS27970	3289257..3290288	-	1032	WP_157787303.1	phage replisome organizer	-
FORC29_RS17535	3290357..3290782	-	426	WP_000693925.1	Rha family transcriptional regulator	-
FORC29_RS17540	3290766..3291041	-	276	WP_047083516.1	helix-turn-helix domain-containing protein	-
FORC29_RS17545	3291149..3291649	+	501	WP_033812093.1	helix-turn-helix transcriptional regulator	-
FORC29_RS17550	3291667..3291858	+	192	WP_033812094.1	hypothetical protein	Antitoxin
FORC29_RS17555	3291858..3292148	+	291	WP_033812095.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC29_RS17560	3292417..3292569	+	153	WP_000380319.1	DUF1391 family protein	-
FORC29_RS27975	3292725..3292982	+	258	WP_077637644.1	hypothetical protein	-
FORC29_RS17570	3293063..3293248	-	186	WP_024213805.1	hypothetical protein	-
FORC29_RS17575	3293793..3293981	+	189	WP_000450222.1	cell division inhibitor	-
FORC29_RS17580	3293978..3294166	+	189	WP_086163675.1	DUF1482 family protein	-
FORC29_RS17585	3294259..3296661	+	2403	WP_088888718.1	exonuclease	-
FORC29_RS17590	3296728..3296979	+	252	WP_000273163.1	DUF4224 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11192.05 Da
• Isoelectric Point: 9.9854

>T58066 WP_033812095.1 NZ_CP013185:3291858-3292148 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLTEHPYLYPPSERVPSLREIVTHPNYIILYRVTTSNIE
IVNVVHSRRQYPNKSC

Nucleotide

• Length: 291 bp

>T58066 NZ_CP013185:3291858-3292148 [Escherichia coli]
ATGCTGCCCATTTTATGGCTACCTTCTGCACGTGATGATTTACGTCAGATAGTAGCCTATATTGCTAAAGAAAACCCTCC
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTACTGAACACCCTTATCTGTACCCACCGAGCG
AAAGAGTTCCAAGTCTTCGAGAGATAGTGACTCATCCTAACTACATAATACTTTACCGAGTAACAACATCTAACATCGAG
ATCGTAAATGTAGTTCACTCACGAAGGCAGTATCCAAACAAAAGCTGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 6993.76 Da
• Isoelectric Point: 4.0270

>AT58066 WP_033812094.1 NZ_CP013185:3291667-3291858 [Escherichia coli]
MSTALSPIISEFETVEQENSYNEWLRAKVAASLADPRSGIPHDEVMAEMENLIAQLATTNRSE

Nucleotide

• Length: 192 bp

>AT58066 NZ_CP013185:3291667-3291858 [Escherichia coli]
ATGAGTACAGCCCTTTCCCCGATAATTTCTGAATTTGAAACGGTCGAACAAGAAAACAGCTATAACGAATGGTTGCGAGC
CAAAGTGGCAGCAAGCCTCGCAGATCCCCGTTCTGGAATTCCGCATGATGAAGTAATGGCTGAAATGGAAAACCTTATTG
CTCAATTAGCTACAACGAACAGGAGTGAGTAA