Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1421954..1422579 Replicon chromosome
Accession NZ_CP013185
Organism Escherichia coli strain FORC_029

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag FORC29_RS07390 Protein ID WP_000911329.1
Coordinates 1422181..1422579 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag FORC29_RS07385 Protein ID WP_000450524.1
Coordinates 1421954..1422181 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC29_RS07360 1417756..1418226 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
FORC29_RS07365 1418226..1418798 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
FORC29_RS07370 1418944..1419822 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
FORC29_RS07375 1419839..1420873 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
FORC29_RS07380 1421086..1421799 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
FORC29_RS07385 1421954..1422181 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
FORC29_RS07390 1422181..1422579 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
FORC29_RS07395 1422726..1423589 + 864 WP_001267498.1 neutral zinc metallopeptidase -
FORC29_RS07400 1423604..1425619 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
FORC29_RS07405 1425693..1426391 + 699 WP_000679823.1 esterase -
FORC29_RS07410 1426501..1426701 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T58054 WP_000911329.1 NZ_CP013185:1422181-1422579 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T58054 NZ_CP013185:1422181-1422579 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT58054 WP_000450524.1 NZ_CP013185:1421954-1422181 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT58054 NZ_CP013185:1421954-1422181 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References