Detailed information of TA system TA058042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 300153..300766
• Replicon: chromosome
• Accession: NZ_CP013184
• Organism: Pseudomonas protegens strain H78

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9LBG9
• Locus tag: H78_RS01345
• Protein ID: WP_041115175.1
• Coordinates: 300153..300335 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H78_RS01350
• Protein ID: WP_171820684.1
• Coordinates: 300332..300766 (+)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H78_RS01325	295891..297852	+	1962	WP_161466434.1	choline transporter BetT	-
H78_RS01330	297862..298119	-	258	WP_015633747.1	hypothetical protein	-
H78_RS01335	298172..298912	-	741	WP_019094920.1	SDR family oxidoreductase	-
H78_RS01340	299045..299935	+	891	WP_011058633.1	LysR family transcriptional regulator	-
H78_RS01345	300153..300335	+	183	WP_041115175.1	type II toxin-antitoxin system HicA family toxin	Toxin
H78_RS01350	300332..300766	+	435	WP_171820684.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H78_RS01355	300779..301825	-	1047	WP_077933197.1	LacI family DNA-binding transcriptional regulator	-
H78_RS01360	302031..304289	+	2259	WP_077933198.1	TonB-dependent receptor	-
H78_RS01365	304433..305761	+	1329	WP_077933199.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (14-138)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6866.97 Da
• Isoelectric Point: 10.8547

>T58042 WP_041115175.1 NZ_CP013184:300153-300335 [Pseudomonas protegens]
MRSREMISRIEADGWYLVAVKGSHHQYKHPFKPGRVTIRHPDTDLPKGTLNSILKQAGLK

Nucleotide

• Length: 183 bp

>T58042 NZ_CP013184:300153-300335 [Pseudomonas protegens]
ATGCGCAGTCGGGAAATGATCAGCAGGATCGAGGCGGATGGTTGGTACTTGGTGGCCGTCAAGGGCAGTCACCATCAGTA
CAAGCATCCTTTCAAGCCGGGCCGCGTGACCATCAGGCACCCGGATACGGATCTGCCCAAAGGCACGCTCAACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 16003.82 Da
• Isoelectric Point: 4.3449

>AT58042 WP_171820684.1 NZ_CP013184:300332-300766 [Pseudomonas protegens]
MSLFLNEESNDMKFPVVLHKDADSEYGVTVPDVPGCHSAGPTVSLAFENIKEALSLHYEGLVADGEPLPQVHEIDDHLDN
PDYAGGVWGVVEFDVTPYFGKSVRFNATLPEQLLERIDQRVERDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 435 bp

>AT58042 NZ_CP013184:300332-300766 [Pseudomonas protegens]
ATGAGCCTGTTTCTGAACGAGGAGTCCAACGACATGAAATTCCCGGTGGTTCTGCACAAGGATGCCGACTCCGAATATGG
GGTGACAGTTCCGGATGTACCGGGCTGCCATTCTGCCGGCCCGACGGTTTCCCTGGCGTTCGAGAACATCAAGGAAGCGC
TGTCCCTGCACTACGAGGGCCTGGTGGCGGACGGCGAGCCCTTGCCCCAGGTGCATGAGATTGACGATCACCTGGACAAC
CCCGACTACGCCGGTGGCGTATGGGGCGTGGTGGAGTTCGACGTGACGCCCTACTTCGGCAAGTCGGTGCGCTTCAACGC
CACCCTGCCGGAGCAATTGCTGGAGCGCATCGACCAGAGGGTCGAACGCGATCAGCGCTACCGTTCGCGTTCCGGGTTCC
TCGCCGCTGCGGCGCTGCGCGAGCTCTCTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LBG9