Detailed information of TA system TA057971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 8943670..8945207
• Replicon: chromosome
• Accession: NZ_CP013127
• Organism: Bradyrhizobium diazoefficiens strain USDA 122

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A837C678
• Locus tag: BD122_RS42270
• Protein ID: WP_049832575.1
• Coordinates: 8943670..8944593 (-)
• Length: 308 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: BD122_RS42275
• Protein ID: WP_074077775.1
• Coordinates: 8944590..8945207 (-)
• Length: 206 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BD122_RS42240	8938738..8939907	+	1170	WP_039187218.1	site-specific integrase	-
BD122_RS42245	8939942..8940460	+	519	WP_131234233.1	hypothetical protein	-
BD122_RS42250	8940476..8941189	+	714	WP_131234232.1	hypothetical protein	-
BD122_RS42255	8941266..8941472	+	207	WP_028175471.1	helix-turn-helix domain-containing protein	-
BD122_RS42260	8941504..8942430	+	927	WP_157862142.1	toprim domain-containing protein	-
BD122_RS42265	8942433..8943515	+	1083	WP_049832576.1	AAA family ATPase	-
BD122_RS42270	8943670..8944593	-	924	WP_049832575.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
BD122_RS42275	8944590..8945207	-	618	WP_074077775.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
BD122_RS42280	8945456..8949514	+	4059	WP_028175467.1	helix-turn-helix domain-containing protein	-
BD122_RS42285	8949641..8950198	+	558	WP_074077613.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	8939942..8961076	21134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-276)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (12-56)

Sequences

Toxin

Protein

• Length: 308 a.a.
• Molecular weight: 34339.39 Da
• Isoelectric Point: 4.9777

>T57971 WP_049832575.1 NZ_CP013127:c8944593-8943670 [Bradyrhizobium diazoefficiens]
MIGKPLKDPGASIRQRLLNHAKEHGDEYQRILTRYAIERLLFRLSQTEARERYVLKGAMLFVTWPEQVFRPTGDLDLLGQ
GDPDPAAIIDLFTLICQVEAPADGIIFDPSTLQVEAVREEEKYQGVRLSLRGRLGTAVIPVQVDIGFGDSVHPAPSRNNF
PSLLPDLPAADVLMYPPETVIAEKFEAMIRFGEANGRIKDFHDIWVTARTFAFNLSTLVEAVSGTLQRRETAFPTEMPVA
LTSQFAEMADKQALWTGFLRRNPPTLPPPPLGDLLAELRRFFEPVLAALPLPESATGRWNPDRGAWE

Nucleotide

• Length: 924 bp

>T57971 NZ_CP013127:c8944593-8943670 [Bradyrhizobium diazoefficiens]
ATGATCGGCAAGCCGCTCAAAGATCCCGGTGCTTCGATCCGTCAGCGCCTGCTCAACCACGCCAAGGAGCACGGCGACGA
GTATCAACGGATTCTGACGCGCTACGCGATTGAGCGGTTGCTTTTCAGACTAAGCCAAACCGAGGCGCGCGAGCGATACG
TCCTCAAGGGCGCGATGCTCTTCGTGACTTGGCCCGAACAAGTGTTTCGGCCCACAGGTGATCTTGACCTTCTTGGGCAA
GGGGATCCCGATCCAGCTGCCATCATCGATCTCTTCACCCTTATCTGCCAGGTCGAGGCGCCGGCGGATGGCATCATCTT
CGACCCCTCTACGCTGCAGGTGGAGGCGGTCCGCGAGGAGGAAAAATATCAGGGTGTCAGACTCTCCCTGAGGGGACGTC
TTGGCACGGCGGTGATCCCTGTCCAGGTGGACATCGGGTTTGGGGACAGTGTTCATCCCGCGCCCAGCCGGAACAATTTC
CCCAGCCTGCTGCCGGACCTGCCGGCGGCGGACGTCCTGATGTACCCGCCCGAGACAGTAATTGCAGAGAAATTCGAAGC
GATGATCCGCTTTGGCGAAGCGAACGGCAGGATCAAAGATTTTCACGATATCTGGGTCACGGCGCGAACCTTCGCTTTCA
ACCTCTCTACGCTGGTCGAGGCGGTTAGCGGTACCCTGCAAAGGCGGGAAACCGCGTTCCCGACGGAGATGCCGGTGGCG
CTGACGTCTCAGTTCGCCGAGATGGCCGACAAACAAGCGCTATGGACAGGCTTTCTGCGCCGCAATCCGCCCACGCTGCC
GCCGCCTCCATTGGGCGACTTGTTGGCGGAGCTGCGGCGGTTCTTCGAACCCGTGCTTGCGGCTCTTCCTTTGCCGGAAA
GCGCGACCGGTCGTTGGAATCCGGACCGCGGCGCCTGGGAATAG

Antitoxin

Protein

• Length: 206 a.a.
• Molecular weight: 22770.44 Da
• Isoelectric Point: 10.4747

>AT57971 WP_074077775.1 NZ_CP013127:c8945207-8944590 [Bradyrhizobium diazoefficiens]
MTQRHVLPYRERALNLVRARGIARARDFKAAGIPLVYLKRLTDTGELVRLGRGLYQDPDRVGEDVAHNLAEAARAVPNGV
VSLASALRYHGLTTQLPHAVWLTIPHKARTPKAIGFPLEIVRATGDVMTAGVEHVQVEGVDVPIYGVAKTIADCFKHRRH
IGEDVAIEALRDALRLRKTTVSDVMRYAEIDRVAERIRPVLKALQ

Nucleotide

• Length: 618 bp

>AT57971 NZ_CP013127:c8945207-8944590 [Bradyrhizobium diazoefficiens]
ATGACCCAACGTCATGTGCTGCCTTATCGCGAGCGCGCCCTCAATCTCGTTCGGGCTCGAGGCATTGCCCGCGCCCGCGA
CTTCAAAGCCGCGGGTATCCCCCTTGTTTACCTGAAGCGACTGACAGACACAGGTGAACTCGTTCGACTTGGAAGAGGGC
TCTACCAAGATCCGGATCGAGTCGGAGAAGATGTCGCTCATAATCTTGCGGAAGCCGCACGGGCCGTTCCCAACGGAGTC
GTTAGCCTTGCCAGTGCGCTCCGGTACCACGGGTTGACGACCCAGCTGCCGCATGCCGTCTGGCTGACCATTCCGCACAA
AGCTAGAACTCCGAAGGCCATTGGCTTCCCGTTGGAAATCGTCCGCGCCACAGGCGATGTGATGACAGCTGGCGTCGAGC
ATGTGCAGGTCGAAGGCGTCGACGTCCCCATATACGGCGTAGCTAAAACCATCGCCGATTGCTTCAAGCACCGTCGTCAT
ATCGGGGAGGACGTCGCGATCGAGGCACTCCGCGATGCGCTCCGCCTGCGGAAGACTACAGTGAGCGATGTCATGAGGTA
CGCCGAGATCGATCGTGTGGCCGAGAGAATCCGCCCCGTTCTTAAGGCGCTTCAATGA

Structures

Toxin

• AlphaFold DB: A0A837C678