Detailed information of TA system TA057963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 107451..108097
• Replicon: plasmid pPME5
• Accession: NZ_CP013125
• Organism: Pseudomonas mendocina S5.2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DW68_RS24400
• Protein ID: WP_017362343.1
• Coordinates: 107451..107867 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DW68_RS24405
• Protein ID: WP_017362342.1
• Coordinates: 107867..108097 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DW68_RS24360	103304..103666	-	363	WP_155718745.1	hypothetical protein	-
DW68_RS24365	103822..104391	-	570	WP_017362350.1	hypothetical protein	-
DW68_RS24370	104427..104741	-	315	WP_017362349.1	hypothetical protein	-
DW68_RS24375	104804..105058	-	255	WP_017362348.1	hypothetical protein	-
DW68_RS24385	105382..105615	-	234	WP_172825372.1	hypothetical protein	-
DW68_RS24390	105727..106209	-	483	WP_017362345.1	hypothetical protein	-
DW68_RS25845	106223..106531	-	309	WP_155718739.1	hypothetical protein	-
DW68_RS24395	106587..107393	-	807	WP_017362344.1	ATP-grasp domain-containing protein	-
DW68_RS24400	107451..107867	-	417	WP_017362343.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DW68_RS24405	107867..108097	-	231	WP_017362342.1	antitoxin	Antitoxin
DW68_RS24410	108305..108562	+	258	WP_017362341.1	nicotinamide mononucleotide transporter	-
DW68_RS24415	108593..109114	+	522	WP_017362340.1	DUF262 domain-containing protein	-
DW68_RS24420	109417..110205	+	789	WP_017362339.1	hypothetical protein	-
DW68_RS24425	110209..110490	+	282	WP_051923039.1	hypothetical protein	-
DW68_RS24430	110538..110747	+	210	WP_017362337.1	hypothetical protein	-
DW68_RS24435	110820..111344	+	525	WP_017362336.1	hypothetical protein	-
DW68_RS24440	111356..111646	+	291	WP_153606264.1	hypothetical protein	-
DW68_RS24445	111686..112405	+	720	WP_017362334.1	phospholipase D family protein	-
DW68_RS24450	112420..112896	+	477	WP_155718746.1	N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..252328	252328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15200.48 Da
• Isoelectric Point: 6.7121

>T57963 WP_017362343.1 NZ_CP013125:c107867-107451 [Pseudomonas mendocina S5.2]
MLKYMLDTNICIFTIKNRPAQVREAFKRAHGQLCISTVTLMELIYGAEKSASPERNLADIEGFAARLEVLAYDGDAAVHT
GQLRAELAKAGTPIGPYDQMIAGHARSQGLILVTINRREFDLAPGLRVEDWVTSGQQL

Nucleotide

• Length: 417 bp

>T57963 NZ_CP013125:c107867-107451 [Pseudomonas mendocina S5.2]
ATGTTGAAGTACATGCTTGATACGAACATCTGCATCTTCACCATCAAGAACAGGCCCGCGCAGGTGCGGGAGGCGTTCAA
GCGTGCCCACGGCCAGCTGTGTATCAGCACTGTGACTTTGATGGAGTTGATCTACGGGGCGGAGAAGTCGGCTAGCCCGG
AGCGGAACCTGGCAGACATCGAGGGCTTTGCCGCTCGCCTGGAAGTATTGGCGTACGACGGCGATGCCGCTGTGCATACC
GGCCAACTCCGCGCCGAGCTTGCCAAAGCCGGAACCCCCATAGGTCCCTATGACCAGATGATCGCCGGGCATGCGCGCTC
GCAGGGCCTGATCCTGGTGACGATCAACCGTCGAGAGTTCGACCTAGCACCTGGGCTTAGGGTCGAGGATTGGGTGACCT
CTGGCCAGCAGTTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8475.37 Da
• Isoelectric Point: 4.5742

>AT57963 WP_017362342.1 NZ_CP013125:c108097-107867 [Pseudomonas mendocina S5.2]
VEKGSVFQSNRSQAVRLPKAAALPDDVKRVDVVSIGRTRIIAPAGESWDSWFEAEGVTRDYMSDRDQPADQEREAL

Nucleotide

• Length: 231 bp

>AT57963 NZ_CP013125:c108097-107867 [Pseudomonas mendocina S5.2]
GTGGAAAAGGGCTCAGTTTTCCAAAGCAACCGCAGCCAGGCCGTGAGGCTACCGAAGGCCGCCGCGCTGCCCGACGACGT
GAAGCGCGTCGATGTGGTTTCTATTGGTAGGACCCGGATCATCGCCCCGGCCGGCGAGTCCTGGGATAGCTGGTTCGAGG
CCGAAGGCGTCACTCGCGACTACATGTCAGACCGCGACCAGCCGGCCGACCAAGAGCGCGAGGCGCTCTGA