Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4846417..4847035 Replicon chromosome
Accession NZ_CP013112
Organism Escherichia coli strain YD786

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ASE18_RS24010 Protein ID WP_001291435.1
Coordinates 4846417..4846635 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ASE18_RS24015 Protein ID WP_000344800.1
Coordinates 4846661..4847035 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASE18_RS23980 4841661..4842014 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ASE18_RS23985 4842056..4843606 - 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ASE18_RS23990 4843770..4844240 - 471 WP_000136192.1 YlaC family protein -
ASE18_RS23995 4844356..4844601 - 246 Protein_4563 maltose O-acetyltransferase -
ASE18_RS24000 4844614..4845822 - 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -
ASE18_RS24005 4845931..4846245 - 315 Protein_4565 maltose O-acetyltransferase -
ASE18_RS24010 4846417..4846635 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ASE18_RS24015 4846661..4847035 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ASE18_RS24020 4847581..4850730 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
ASE18_RS24025 4850753..4851946 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4844614..4845822 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T57939 WP_001291435.1 NZ_CP013112:c4846635-4846417 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T57939 NZ_CP013112:c4846635-4846417 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT57939 WP_000344800.1 NZ_CP013112:c4847035-4846661 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT57939 NZ_CP013112:c4847035-4846661 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References