Detailed information of TA system TA057919

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 663211..664051
• Replicon: chromosome
• Accession: NZ_CP013112
• Organism: Escherichia coli strain YD786

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: ASE18_RS03300
• Protein ID: WP_000854686.1
• Coordinates: 663668..664051 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M0YJ34
• Locus tag: ASE18_RS03295
• Protein ID: WP_001360163.1
• Coordinates: 663211..663591 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASE18_RS03270	660327..661145	+	819	WP_001234732.1	DUF945 domain-containing protein	-
ASE18_RS03275	661237..661722	+	486	WP_000214307.1	antirestriction protein	-
ASE18_RS03280	661738..662214	+	477	WP_001186726.1	RadC family protein	-
ASE18_RS03285	662277..662498	+	222	WP_000692298.1	DUF987 domain-containing protein	-
ASE18_RS03290	662517..663200	+	684	WP_000086769.1	hypothetical protein	-
ASE18_RS03295	663211..663591	+	381	WP_001360163.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ASE18_RS03300	663668..664051	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
ASE18_RS03305	664048..664536	+	489	WP_001054233.1	hypothetical protein	-
ASE18_RS03310	664553..664750	+	198	WP_000839253.1	DUF957 domain-containing protein	-
ASE18_RS03315	664835..665677	+	843	WP_001274547.1	DUF4942 domain-containing protein	-
ASE18_RS03325	666148..667086	+	939	WP_000351311.1	glyoxylate/hydroxypyruvate reductase GhrA	-
ASE18_RS03330	667141..667878	+	738	WP_000283664.1	zinc-binding phosphatase	-
ASE18_RS03335	667902..668456	+	555	WP_001001902.1	molecular chaperone YcdY	-
ASE18_RS03340	668558..669049	+	492	WP_001297187.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T57919 WP_000854686.1 NZ_CP013112:663668-664051 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T57919 NZ_CP013112:663668-664051 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13950.74 Da
• Isoelectric Point: 5.0823

>AT57919 WP_001360163.1 NZ_CP013112:663211-663591 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCIVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT57919 NZ_CP013112:663211-663591 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCATCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A1M0YJ34