Detailed information of TA system TA057918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1975357..1976027
• Replicon: plasmid C
• Accession: NZ_CP013110
• Organism: Sinorhizobium americanum strain CFNEI 73

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1L3LZT4
• Locus tag: SAMCFNEI73_RS30590
• Protein ID: WP_064252821.1
• Coordinates: 1975357..1975776 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SAMCFNEI73_RS30595
• Protein ID: WP_037382358.1
• Coordinates: 1975773..1976027 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAMCFNEI73_RS30560	1971725..1971988	+	264	WP_064252817.1	hypothetical protein	-
SAMCFNEI73_RS30565	1972090..1973397	+	1308	WP_064252818.1	hypothetical protein	-
SAMCFNEI73_RS30570	1973384..1974175	+	792	WP_064252819.1	ATP-dependent Clp protease proteolytic subunit	-
SAMCFNEI73_RS30575	1974255..1974506	+	252	WP_064252820.1	type II toxin-antitoxin system ParD family antitoxin	-
SAMCFNEI73_RS30580	1974614..1974796	+	183	WP_037382362.1	hypothetical protein	-
SAMCFNEI73_RS30585	1974856..1975116	+	261	WP_037382360.1	hypothetical protein	-
SAMCFNEI73_RS30590	1975357..1975776	-	420	WP_064252821.1	type II toxin-antitoxin system VapC family toxin	Toxin
SAMCFNEI73_RS30595	1975773..1976027	-	255	WP_037382358.1	plasmid stabilization protein	Antitoxin
SAMCFNEI73_RS30600	1976241..1977722	-	1482	WP_064252822.1	trypsin-like peptidase domain-containing protein	-
SAMCFNEI73_RS30605	1978056..1978490	-	435	WP_037382356.1	OsmC family protein	-
SAMCFNEI73_RS30610	1978654..1979877	-	1224	WP_064252823.1	MFS transporter	-
SAMCFNEI73_RS30615	1979995..1980573	+	579	Protein_1780	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	katA / htpB	1..2177502	2177502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14824.06 Da
• Isoelectric Point: 6.4696

>T57918 WP_064252821.1 NZ_CP013110:c1975776-1975357 [Sinorhizobium americanum]
MIVLDTNVVSEAMKPSPDPAVRSWLNEQVAESLYLSSVTMAELLFGVGALPDGRRKKALTETLHGVVELFGDRVLPFDIG
AARHYAELAVRARAAGKGFPTPAGYIAAIAASRGFLVATRDVSPFEAAGLPVVNPWAYQ

Nucleotide

• Length: 420 bp

>T57918 NZ_CP013110:c1975776-1975357 [Sinorhizobium americanum]
ATGATCGTCTTGGATACGAATGTCGTCTCCGAAGCGATGAAACCATCTCCCGATCCGGCCGTGCGCTCCTGGTTGAACGA
ACAGGTCGCTGAAAGCTTGTACCTGTCCAGTGTGACAATGGCCGAACTGCTATTTGGGGTCGGTGCGTTGCCCGATGGTC
GGCGCAAGAAGGCTCTGACCGAAACGCTTCACGGCGTCGTCGAACTGTTTGGTGATCGTGTATTGCCGTTCGACATCGGA
GCTGCCCGGCATTACGCAGAACTGGCCGTGAGAGCGCGAGCGGCGGGGAAGGGATTTCCTACGCCCGCCGGATATATTGC
CGCCATTGCCGCCTCGCGTGGATTCCTCGTCGCGACGCGCGATGTCAGTCCCTTCGAAGCGGCGGGACTTCCCGTCGTGA
ATCCATGGGCCTATCAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9492.85 Da
• Isoelectric Point: 7.2069

>AT57918 WP_037382358.1 NZ_CP013110:c1976027-1975773 [Sinorhizobium americanum]
MAILTVRNVPEEVHRALRVRAATHGRSTEAEVRDILESVVKPAQRVRMGDALAELGRRLGLTNDDFVVFEQVRDKTPAEP
VRFE

Nucleotide

• Length: 255 bp

>AT57918 NZ_CP013110:c1976027-1975773 [Sinorhizobium americanum]
ATGGCCATCTTGACAGTTCGGAATGTACCCGAGGAAGTGCATCGCGCTTTGCGCGTGCGGGCGGCGACGCACGGTCGCAG
CACCGAGGCGGAAGTCCGCGATATTCTGGAAAGTGTGGTGAAGCCCGCGCAGCGCGTTCGCATGGGGGATGCCTTGGCCG
AACTCGGCCGGCGACTGGGCCTGACGAACGACGATTTCGTCGTGTTTGAGCAGGTACGTGACAAGACGCCGGCAGAGCCA
GTGAGGTTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A1L3LZT4